Table 2 Immunoreactive proteins of A.fumigates identified by MALDI-TOF-MS
Spot no. | Accession No. (GenBank) | Organism | Protein name | Peptides matched | Sequence coverage(%) | Mascot score | BLAST score (E-value) | Theoretical pI/Mr(kDa) | Probable functions |
|---|---|---|---|---|---|---|---|---|---|
1A-1H | GI:71001112 | Aspergillus fumigatus Af293 | secreted dipeptidyl peptidase DppV | 26 | 33 | 135 | 1.60E-08 | 5.59/79.7 | Metabolism of dipeptides |
2A-2M | GI:70992029 | Aspergillus fumigatus Af293 | thioredoxin reductase GliT | 20 | 54 | 149 | 6.30E-10 | 5.44/36.2 | Provide self-protection to A. fumigatus |
3 | GI:159123228 | Aspergillus fumigatus A1163 | FAD dependent oxidoreductase, putative | 25 | 44 | 173 | 2.50E-12 | 5.94/51.5 | Oxidoreductase |
4 | GI:70989411 | Aspergillus fumigatus Af293 | fumarylacetoacetate hydrolase FahA | 13 | 37 | 85 | 0.0015 | 5.95/46.9 | Phenylalanine catabolism, Tyrosine catabolism |
5 | GI: 119492487 | Neosartorya fischeri NRRL 181 | aspartyl aminopeptidase | 20 | 40 | 98 | 8.90E-05 | 6.03/53.9 | proteolysis, tissue invasion |
6A-6B | GI: 70992355 | Aspergillus fumigatus Af293 | aldehyde dehydrogenase AldA | 25 | 54 | 171 | 4.00E-12 | 6.30/61.4 | Alcohol metabolism |
7 | GI: 71002030 | Aspergillus fumigatus Af293 | aromatic aminotransferase Aro8 | 19 | 52 | 145 | 3.10E-08 | 5.96/58.3 | Aromatic aminoacid family metabolic process |
8A-8B | GI: 70999466 | Aspergillus fumigatus Af293 | fructose-bisphosphate aldolase, class II | 19 | 62 | 137 | 9.90E-09 | 5.55/39.9 | Glycolysis, Carbohydrate degradation |
9 | GI: 119499942 | Neosartorya fischeri NRRL 181 | G-protein comlpex beta subunit CpcB | 18 | 59 | 130 | 5.00E-08 | 6.06/35.3 | Receptor signaling, intracellular signal transduction pathways, and protein synthesis |
10 | GI: 71001310 | Aspergillus fumigatus Af293 | actin cytoskeleton protein (VIP1) | 13 | 40 | 86 | 0.0013 | 5.93/28.3 | Component of cytoskeleton |
11 | GI: 159129975 | Aspergillus fumigatus A1163 | phytanoyl-CoA dioxygenase family | 15 | 64 | 109 | 6.30E-06 | 6.08/33.7 | Oxidization |
12 | GI: 70988713 | Aspergillus fumigatus Af293 | pectate lyase A | 13 | 44 | 96 | 0.00014 | 6.23/33.8 | Carbohydrate metabolism, cell wall biogenesis/degradation |
13 | GI: 71001408 | Aspergillus fumigatus Af293 | urate oxydase UaZ | 12 | 32 | 80 | 0.0052 | 7.24/34.1 | Metabolism of urate |
14 | GI: 70986899 | Aspergillus fumigatus Af293 | malate dehydrogenase, NAD-dependent | 23 | 70 | 258 | 7.90E-21 | 9.08/35.8 | Cellular carbohydrate metabolic process |
15 | GI: 169764553 | Aspergillus oryzae RIB40 | hypothetical protein | 13 | 41 | 92 | 2.90E-04 | 6.21/35.3 | unknown |
16A-16B | GI: 70982195 | Aspergillus fumigatus Af293 | 3-hydroxybutyryl-CoA dehydrogenase | 15 | 44 | 90 | 0.00052 | 6.33/36.0 | Fatty acid metabolic process |
17 | GI: 121711615 | Aspergillus clavatus NRRL 1 | proteasome component Pre8, putative | 12 | 44 | 80 | 0.0052 | 5.55/30.1 | Proteolysis involved in cellular protein catabolic process |