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Table 2 Coverage, Shannon Index, and LIBSHUFF method calculated using MOTHURa for each methanogen 16S rRNA gene clone library

From: Molecular analysis of methanogenic archaea in the forestomach of the alpaca (Vicugna pacos)

Clone Library

No. of unique OTUs

% OTU coverage

Shannon Index ±

95% confidence limits

LIBSHUFF Methodc

Alpaca 4

3

97.8

2.06 ± 0.15b

P≤ 0.0004

Alpaca 5

5

93.5

2.12 ± 0.14b

P≤ 0.0022

Alpaca 6

2

94.0

1.96 ± 0.15b

P≤ 0.0001

Alpaca 8

3

95.2

1.89 ± 0.16b

P≤ 0.0028

Alpaca 9

6

94.4

2.09 ± 0.17b

P≤ 0.0028

Combined

-

98.4

2.85 ± 0.07b

-

  1. a Schloss et al. [23]
  2. b No significant difference between these values
  3. c LIBSHUFF Method calculated for each pair of methanogen 16S rRNA gene clone libraries (e.g. the comparison of Alpaca 4 against Alpaca 5, Alpaca 6, Alpaca 8, and Alpaca 9 was always significantly different P ≤ 0.0004)
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BMC Microbiology

ISSN: 1471-2180

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