Table 1 Bacterial strains and plasmids used in this study
Strains or plasmids | Genotypes or descriptions | Sources |
|---|---|---|
C. crescentus | ||
LS107 | syn-1000, bla-6, amps derivative of NA1000 | Stephens et al. [45] |
JG1172 | syn-1000 bla-6 ΔfliX | Muir et al. [38] |
SC1032 | flbD198::Tn5 | Ohta et al. [41] |
E. coli | ||
S17-1 | Rp4-2, Tc::Mu, Km::Tn7 | Simon et al. [46] |
BL21(DE3) | F-ompT gal [dcm] [lon] hsdS B (rB- mB-; an E. coli B strain) with DE3, a λ prophage carrying the T7 RNA polymerase gene | Novagen |
Plasmids | ||
pX21b | derivative of pET-21b carrying histidine-tagged FliX under the control of T7 promoter, Apr | Muir & Gober [36] |
pBBR1MCS | broad host range cloning vector, multicopy, Cmr | Kovach et al. [47] |
pZXfliX | derivative of pBBR1MCS carrying PCR generated 894 bp BamHI-HindIII fragment containing fliX gene and its promoter | This study |
pZX71A | derivative of pZXfliX, codon 71 cgcc→gcc | This study |
pZX85K | derivative of pZXfliX, codon 85 ctg→aag | This study |
pZXΔ117-8 | derivative of pZXfliX, deletion of codon 117 and 118 | This study |
pZX130L | derivative of pZXfliX, codon 130 acc→ctg | This study |
pZX136K | derivative of pZXfliX, codon 136 ctg→aag | This study |
pfliX1 | derivative of pBBR1-MCS expressing fliX 1, an allele of fliX carrying a point mutation at the sixteenth codon (R16G), a frame shift at codon 141, and an extended carboxyl terminus of 67 amino acids | Muir et al. [38] |
pfliF/lacZ/290 | fliF-lacZ transcriptional reporter vector, Tcr | Wingrove & Gober [48] |
pfliK/lacZ/290 | fliK-lacZ transcriptional reporter vector, Tcr | Gober & Shapiro [25] |