Genetically altering the expression of neutral trehalase gene affects conidiospore thermotolerance of the entomopathogenic fungus Metarhizium acridum

Table 1 Trehalose concentrations and neutral trehalase activity in wild-type strain compared to over-expression mutants and RNAi mutants

Strains	Trehalose (pg/conidium)*		Neutral trehalase activity (U/mg protein)*
1	7.17 ± 0.93	c	14.28 ± 1.14	c
2	5.04 ± 1.17	e	18.08 ± 1.15	ab
3	6.10 ± 0.22	d	16.43 ± 1.21	b
4	5.91 ± 0.27	de	16.29 ± 1.15	b
5	5.51 ± 0.53	e	16.12 ± 0.96	b
6	9.72 ± 0.14	b	8.82 ± 1.26	d
7	10.76 ± 0.83	a	7.59 ± 0.99	e
8	10.38 ± 0.83	ab	8.33 ± 1.12	de
9	10.57 ± 1.31	ab	8.23 ± 1.39	de

*Means (±SE) of 3 repetitions followed by different lowercase letters in the same column were significantly different at the p < 0.05 level according to the ANOVA table and Tukey's multiple range test. 1: wild-type strain; 2-5: over-expression mutants; 6-9: RNAi mutants.

ISSN: 1471-2180