| Relevant genotype | Source or construction |
---|---|---|
E. coli strains | ||
BW27784 | Δ(araBAD)567 Δ(rhaBAD)568 | Yale E. coli Genetic Stock Center |
 | Δ(araFGH) Φ(ΔaraEpPCP18-araE) | [32] |
BW117N | BW27784 with chromosomally integrated YpTOP1-D117N gene | [10] |
AQ4335 | Δara leu7697 NBRP | NBRP-E. coli at NIG |
FB20344 | MG1655 ydeA::Tn5KAN-I-SceI | U. Wisconsin [34] |
YT103 | AQ4335 ydeA::Tn5KAN-I-SceI | P1(FB20344) × AQ4335, Kanr |
JW1328-1 | Δfnr771::kan | Yale E. coli Genetic Stock Center [35] |
JW1650-1 | ΔpurR746::kan | Yale E. coli Genetic Stock Center [35] |
IFL6 | BW27784 Δ fnr771::kan | P1(JW1328-1) × BW27784, Kanr |
IFL7 | BW27784 Δ purR746::kan | P1(JW1650-1) × BW27784, Kanr |
Plasmids | ||
pAYTOP128 | Mutant derivative of pAYTOP encoding YpTOP1 with G122S, M326V and A383P mutations | [11] |
pCRII | High copy number cloning vector | Invitrogen |
pAQ5 | pCR-XL-TOPO cloning product of E. coli chromosome fragment 2618398-2620765 | This study |
pAQ5-1 | pCR-XL-TOPO carrying upp gene and the intergenic region of upp-purMN | This study |
pAQ5-2 | pCR-XL-TOPO carrying purM gene and the intergenic region of upp-purMN | This study |
pInter | pCR-XL-TOPO carrying the intergenic region of upp-purMN | This study |
pInterD1 | pInter with the FNR binding site deleted | This study |
pInterD2 | pInter with the PurR binding site deleted | This study |