| Sample | |||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 | Combined sequence pool | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | |||||||||
 | V1V2 | V6 | V1V2 | V6 | V1V2 | V6 | V1V2 | V6 | V1V2 | V6 | V1V2 | V6 | V1V2 | V6 | V1V2 | V6 | V1V2 | V6 |
Sampling depth | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | |
Total reads | 78346 | 74067 | 14579 | 18362 | 12629 | 6565 | 4305 | 17474 | 9877 | 5005 | 12645 | 6586 | 8216 | 5692 | 7861 | 6986 | 8234 | 7397 |
Length cutoff1 | 48861 | 45382 | 8479 | 8039 | 8416 | 4752 | 2721 | 13066 | 6253 | 3467 | 10116 | 5074 | 4428 | 3047 | 3967 | 3495 | 4481 | 4442 |
Denoised 2 | 48860 | 45136 | 8479 | 7977 | 8416 | 4703 | 2721 | 13064 | 6253 | 3461 | 10116 | 5057 | 4427 | 3031 | 3967 | 3432 | 4481 | 4411 |
Cleaned 3 | 48452 | 44760 | 8476 | 7969 | 8353 | 4682 | 2720 | 13060 | 6242 | 3459 | 10109 | 5053 | 4361 | 2988 | 3711 | 3138 | 4480 | 4411 |
Unique OTUs | 1354 | 2069 | 61 | 376 | 456 | 328 | 22 | 115 | 116 | 102 | 95 | 81 | 523 | 134 | 322 | 581 | 163 | 538 |
OTUs4 3% | 1209 | 1435 | 52 | 240 | 411 | 254 | 20 | 81 | 101 | 85 | 73 | 63 | 504 | 116 | 300 | 499 | 130 | 338 |
OTUs4 6% | 1092 | 1072 | 50 | 178 | 379 | 210 | 19 | 61 | 92 | 73 | 62 | 51 | 472 | 101 | 270 | 436 | 116 | 244 |
Phyla5 (11) | 10 | 8 | 4 | 4 | 6 | 3 | 1 | 3 | 4 | 4 | 3 | 3 | 3 | 4 | 8 | 7 | 4 | 4 |
Genera5 (45) | 35 | 28 | 8 | 8 | 15 | 10 | 1 | 8 | 10 | 5 | 6 | 4 | 4 | 4 | 19 | 17 | 9 | 8 |
Diversity indices | ||||||||||||||||||
Chao16 (3%) | 1211 | 2469 | 64.75 | 456.36 | 412.62 | 410.33 | 24.5 | 128.83 | 104 | 195.5 | 86.04 | 108.76 | 504.11 | 130.6 | 324.6 | 1121.43 | 250.12 | 835.02 |
Chao1 LCI95 | 1209 | 2286 | 56.13 | 371.05 | 411.36 | 353.85 | 20.97 | 102.95 | 101.7 | 136.49 | 77.88 | 82.43 | 504 | 122.1 | 313.14 | 953.17 | 195.84 | 670.9 |
Caho1 HCI95 | 1216 | 2690 | 91.27 | 597.21 | 418.2 | 498.76 | 40.69 | 185.2 | 112.75 | 322.11 | 107.8 | 170.8 | 506.28 | 148.39 | 346.03 | 1352.03 | 349.14 | 1080.04 |
Shannon index7 (3%) | 2.99 | 3.05 | 0.52 | 1.96 | 1.99 | 1.62 | 0.23 | 0.49 | 1.44 | 1.44 | 0.33 | 0.44 | 3.01 | 1.32 | 3.76 | 4.07 | 2.06 | 3.31 |
Normalized Shannon index (3%) 8 | Â | Â | 0.52 | 1.96 | 1.86 | 1.63 | 0.23 | 0.50 | 1.42 | 1.44 | 0.34 | 0.45 | 2.89 | 1.35 | 3.72 | 4.07 | 2.06 | 3.31 |