Characterization of a Mycobacterium smegmatis uvrAmutant impaired in dormancy induced by hypoxia and low carbon concentration

Table 2 Synthetic oligonucleotides

Name	Sequence (5' - 3')^a	Position of annealing ^b
uvrA-Ms-Y	ctag tctaga gacgtgtccggtgtaggtgt	-180/-160
uvrA-Ms-R	ctag tctaga atgacctggtggatcgactg	+150/+169
uvrA-Tb-F	ctag tctaga cgatgccttgaggatcgtg	-258/-240
uvrA-Tb-R	ctag tctaga gaagatcgaaacccgatacg	+194/+213

^a Underlined is an unpaired tail carrying Xbal restriction site. ^b Position of annealing refers to the uvrA gene sequence, with the first base of the translational initiation codon as +1.

ISSN: 1471-2180