Figure 2From: Characterization of a Mycobacterium smegmatis uvrAmutant impaired in dormancy induced by hypoxia and low carbon concentrationScreening of M. smegmatis mutant library. A) (Left panel) M. smegmatis wild type and ppk mutant were grown in M9 minimal medium supplemented with glucose 0.2% until OD600nm = 1.0, serially diluted up to 10-5 and transferred by using a metal replicator on agar plates. (Right panel) After incubation at 37°C for 4-5 days for aerobic cultures, or incubation for 2 weeks in an AnaeroGen gas pack system at 37°C followed by incubation under aerobic condition at 37°C for 4-5 days, plates were compared. B) Individual screening of 6 selected mutants. Each clone was grown in M9 minimal medium supplemented with glucose 0,2% until OD600nm = 1.0, serially diluted up to 10-5 and transferred by using a metal replicator on agar plates. Clones 1, 3 and 6 were considered as moderately affected clones. Clone 2 was considered as severely affected. ND = Non Diluted cultureBack to article page