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Table 3 Vectors and interaction summary

From: The protein interaction map of bacteriophage lambda

Vector pair(s)

Fusions proteins

Interactions*

pDEST22/pDEST32

N/N (N-terminal fusions)

8

pGADT7g/pGBKT7g

N/N (N-terminal fusions)

44

pGBKT7g/pGADCg

N/C (N-terminal/C-terminal fusions)

39

pGBKCg/pGADCg

C/C (C-terminal/C-terminal fusions)

18

pGBKCg/pGADT7g

C/N (C-terminal/N-terminal fusions)

26

  1. * Redundant, i.e. some interactions are found with multiple vectors.
  2. Fusion proteins indicate the location of the DNA-binding (DBD) and activation domains (AD), respectively, of each vector pair. For instance, the pDEST vectors both have the DBD and AD fused at the N-terminus of the bait and prey protein. Vectors are listed as bait/prey pairs.
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BMC Microbiology

ISSN: 1471-2180

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