Figure 5From: Physiologic cold shock of Moraxella catarrhalisaffects the expression of genes involved in the iron acquisition, serum resistance and immune evasionCold shock results in upregulation of UspA2 and increases the binding of vitronectin on the surface of M. catarrhalis. Representative flow-cytometric profiles of M. catarrhalis strains O35E, O35E.uspA1 and O35E.uspA2 after exposure at 26°C (gray) or at 37°C (black) show cold shock-dependent UspA1/A2 upregulation (A) and UspA2-dependent binding to vitronectin (C). The dotted line represents the negative control (bacteria incubated with secondary antibodies only). The mean fluorescence intensity ± 1 standard deviation for 2 experiments performed is shown (B and D). *, p < 0.05 for 26°C versus 37°C (one-way analysis of variance).Back to article page