Figure 4

Neutrophil recruitment and chemokine expression in the lungs following infection with the galU mutant. C57Bl/6J mice (4/group) were infected intranasally with 5 × 10⁴ CFU (or 50 × LD₅₀) of either the WT or galU mutant strain of FT and BALF was collected from individual mice at 24, 48, 72 and 96 hours post-infection. Flow cytometric analyses were performed on the cells recovered from BALF to determine the numbers of neutrophils at each timepoint. Statistical analyses were performed via two-way ANOVA with a Bonferroni multiple comparisons post-test and statistically significant differences (P < 0.05) are indicated (*) (Panel A). The concentrations of KC, G-CSF, MIG, and IL-10 (Panel A) and TNF-α, MIP-1α, MIP-1β, MIP-2, and MCP-1 (Panel B) in BALF at the 24 and 48 hour time points, respectively, were determined using a Luminex multiplex kit. Statistical analyses were performed using unpaired t tests. Statistically significant differences (*) and p values are indicated in each panel. The data shown is representative of three independent experiments of similar design.