From: Phylogeography of Francisella tularensis subspecies holarctica from the country of Georgia
SNP | SCHU S4 position | Genome SNP state (D/A)a | Melt MAMA primerc | Melt-MAMA primer sequencesd | Primer conc. (μM) | Annealing temp. (°C) | Melting Tm (°C) |
---|---|---|---|---|---|---|---|
B.Br.026 | 1484645 | A/C | D | GAAACTTATTTGTTCCTAAGACAGTGACAcTA | 0.800 | 55 | 73.1 |
 |  |  | A | ggggcggggcggggcAAACTTATTTGTTCCTAAGACAGTGACAgTC | 0.200 |  | 79.7 |
 |  |  | C | GCATTGAGTTTGACAGGGTTGC | 0.200 |  |  |
B.Br.027 | 1329722 | T/Gb | D | ggggcggggcggggcggggcCATGCCAGGCACTACAATTGATAGTaTA | 0.200 | 55 | 78.2 |
 |  |  | A | TGCCAGGCACTACAATTGATAGTtTC | 1.000 |  | 73.6 |
 |  |  | C | TATACTTCTGACCATGGCGTTCAAAT | 0.200 |  |  |
B.Br.028 | 212729 | T/G | D | ggggcggggcggggcggggcAAATTAGTTCAAATGTTAAATTTGATcCT | 0.200 | 55 | 75.8 |
 |  |  | A | AAATTAGTTCAAATGTTAAATTTGATaCG | 0.200 |  | 67.7 |
 |  |  | C | CAAAATAAATCCCGTTGAGAATAGAA | 0.200 |  |  |
B.Br.029 | 1185519 | A/G | D | ggggcggggcggggcggggcTGCTTAATCTCATTGACTAGCTGTGgTA | 0.200 | 55 | 78 |
 |  |  | A | TGCTTAATCTCATTGACTAGCTGTGaTG | 1.000 |  | 70 |
 |  |  | C | ACAAAGTTGAAACTATCGAGCATAAATC | 0.200 |  |  |
B.Br.030 | 928335 | T/G | D | ggggcggggcggggcggggcTGTTGGGTCAAAGAGAGAAGTgTT | 0.200 | 55 | 78.2 |
 |  |  | A | ATTGTTGGGTCAAAGAGAGAAGTaTG | 0.200 |  | 70 |
 |  |  | C | GCCACCAAAGAATACAGAGTAGTCAT | 0.200 |  |  |
B.Br.031 | 1634565 | A/G | D | ggggcggggcggggcggggcGCACCAATCGTATCTAATTGATcCA | 0.400 | 55 | 79 |
 |  |  | A | GCACCAATCGTATCTAATTGATtCG | 0.200 |  | 70 |
 |  |  | C | AACTTTGCTAAAACAAATGCTGTTGC | 0.200 |  |  |
B.Br.032 | 283540 | A/Gb | D | ggggcggggcggggcggggcTGCTAAACCTACAGTAATCAGAAGTATtAT | 0.200 | 55 | 72 |
 |  |  | A | TGCTAAACCTACAGTAATCAGAAGTATcAC | 0.600 |  | 68.4 |
 |  |  | C | GCTAAATTTTAGTAAGATAAAAAGTGTAAGTAGTG | 0.200 |  |  |