Figure 2From: Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexaconfers enhanced adhesion to cultured cells and fibronectinSurface localization of LigA and LigB. Surface immunofluorescence assay was performed with L. biflexa wild-type strain (Patoc wt), and ligA- (Patoc ligA), and ligB- (Patoc ligB) L. biflexa transformants. Strains were labeled with normal rabbit serum (control) and antibodies against LigA (LigANI), LigB (LigBNI), whole leptospires, and GroEL. A DAPI counterstain was used to document the presence of leptospires. A photomicrograph is shown from one of three representative experiments.Back to article page