| Intra-assay 1 | Inter-assay 2 | ||||||
---|---|---|---|---|---|---|---|---|
 | C. coli | C. jejuni | C. coli | C. jejuni | ||||
Genome copy number | CVc(%) | Ct range | CVj(%) | Ct range | CVc(%) | Ct range | CVj(%) | Ct range |
10 8 | 2.27 | 14.18-15.25 | 5.63 | 14.18-17.15 | 4.89 | 13.86-16.11 | 1.94 | 14.30-15.01 |
10 7 | 1.33 | 16.63-17.71 | 0.95 | 17.55-18.21 | 4.69 | 16.33-17.88 | 0.83 | 17.86-18.27 |
10 6 | 1.99 | 20.05-20.78 | 1.13 | 21.02-21.81 | 3.42 | 19.29-21.80 | 1.37 | 21.15-22.04 |
10 5 | 1.60 | 23.32-24.63 | 0.57 | 24.15-24.69 | 4.08 | 23.22-25.55 | 0.67 | 24.01-24.48 |
10 4 | 0.81 | 26.92-28.07 | 0.35 | 26.56-26.91 | 1.52 | 26.23-28.48 | 2.65 | 26.64-28.30 |
10 3 | 1.28 | 30.44-31.28 | 0.53 | 30.11-30.69 | 1.90 | 29.70-31.37 | 1.99 | 28.60-30.85 |
10 2 | 1.22 | 33-37-34.82 | 0.40 | 33.66-34.05 | 2.46 | 33.80-35.78 | 1.39 | 33.62-34.60 |
10 1 | 0.87 | 37.29-38.66 | 2.21 | 35.65-37.77 | 3.10 | 37.10-38.91 | 2.21 | 36.11-37.43 |
CFU/g of faeces | CV c (%) | Ct range | CV j (%) | Ct range | CV c (%) | Ct range | CV j (%) | Ct range |
2 × 10 8 | 3.23 | 17.22-18.35 | 2.28 | 18-74-19.81 | - | - | - | - |
2 × 10 7 | 1.33 | 20.60-21.15 | 2.53 | 20.57-22.02 | 0.75 | 19.54-19.88 | 1.21 | 21.65-22.27 |
2 × 10 6 | 1.89 | 24.08-24.97 | 0.91 | 24.13-24.62 | 2.37 | 23.51-24.85 | 0.70 | 24.15-24.60 |
2 × 10 5 | 1.15 | 27.23-28.38 | 1.40 | 27.02-28.45 | 0.57 | 26.40-26.79 | 1.46 | 27.04-28.69 |
2 × 10 4 | 2.20 | 28.28-29.75 | 1.98 | 30.13-31.80 | 2.58 | 28.00-29.90 | 2.10 | 30.7-32.31 |
2 × 10 3 | 4.40 | 32.20-33.77 | 1.62 | 34.61-35.96 | 2.07 | 32.00-33.22 | 1.80 | 34.48-36.45 |
2 × 10 2 | 4.38 | 34.61-37.78 | 1.76 | 38.04-39.37 | 1.64 | 35.35-36.56 | 1.92 | 37.34-39.03 |