Study of the functionality of the Helicobacter pylori trans-translation components SmpB and SsrA in an heterologous system

Table 2 E. coli strain used in this study.

Strains	ssrA and smpB alleles	Plasmids [antibiotic resistance]
MG1655 pILL2150	smpB^EcssrA^Ec/pILL2150	multicopy [Cm]
MG1655 pEXT21	smpB^Ec ssrA^Ec/pEXT21>	low copy [Sp]
MG1655 ΔsmpB pILL2150	ΔsmpB^Ec ssrA^Ec/pILL2150	multicopy [Cm]
MG1655 ΔsmpB pILL786	ΔsmpB^Ec ssrA^Ec/pILL2150 with smpB^Hp	multicopy [Cm]
MG1655 ΔssrA pILL2150	smpB^EcΔssrA^Ec/pILL2150	multicopy [Cm]
MG1655 ΔssrA pILL2334	smpB^EcΔssrA^Ec/pILL2334 with ssrA^Ec-WT	multicopy [Cm]
MG1655 ΔssrA pILL788	smpB^EcΔssrA^Ec/pILL2150 with ssrA^Hp-WT	multicopy [Cm]
MG1655 ΔssrA pILL2318	smpB^EcΔssrA^Ec/pEXT21 with ssrA^Hp-WT	low copy [Sp]
MG1655 ΔssrA pILL791	smpB^EcΔssrA^Ec/pILL2150 with ssrA^Hp-DD	multicopy [Cm]
MG1655 ΔssrA pILL792	smpB^EcΔssrA^Ec/pILL2150 with ssrA^Hp-resume	multicopy [Cm]
MG1655 ΔssrA pILL793	smpB^EcΔssrA^Ec/pILL2150 with ssrA^Hp-wobble	multicopy [Cm]
MG1655 ΔssrA pILL794	smpB^EcΔssrA^Ec/pILL2150 with ssrA^Hp-smpB	multicopy [Cm]
MG1655 ΔssrA pILL2328	smpB^EcΔssrA^Ec/pILL2150 with ssrA^Hp-STOP	multicopy [Cm]

The doubling time of each E. coli strain was calculated from growth curves performed in LB medium at 37°C with chloramphenicol [Cm] 100 μg/ml or with spectinomycin [Sp] 100 μg/ml.

ISSN: 1471-2180