Skip to main content

Table 1 Primers used in study.

From: Genetic analysis of the Staphylococcus epidermidis Macromolecular Synthesis Operon: Serp1129 is an ATP binding protein and sigA transcription is regulated by both σA- and σB-dependent promoters

Primer Number

5'-3' Sequence

Description

942

GCATTTGAAGCGGGTTTGTTATC

Forward dnaG

943

ATTGACTAAAGAAAGGTCTC

Reverse dnaG

1035

GGCTATACTCACGATGTCTGG

Forward serp1130/RT-PCR primer

1036

TTTATATTATCCATGTACTTACCACC

Reverse serp1130

670

ACATGTGTTTATTTACTTTTCAACCCGC

RT-PCR primer

672

ATTGTTGCCTCTGATTCAATTGGTG

Forward serp1129/RT-PCR primer

673

TGCATATCTAGCTTTGTCACCTAATCC

Reverse serp1129/RT-PCR primer

675

TTTAACAATTCGTATGCTGCATGG

Reverse sigA

760

CTTGCTTTCGAATAAATTTACGTGC

RT-PCR primer

674

AAGCAAACAATTGATCCGACTTTAACAC

RT-PCR primer/forward sigA

677

GGATTTTATATCACGCAGTGCTTCTAAC

RT-PCR primer

940

ATTATTATCAAAAAATTGTGCC

RT-PCR primer

1135

TTTAAATACATACGCACTGGGTC

RT-PCR primer

1178

CATCGTGAGTATAGCCAAGTCAGGACG

Primer extension 5' end

1196

TCAGCTATATGCTCGCCCGTGATAG

Primer extension 5' end

1194

CTTGGTTGTCAGACATGAAAAGGCCT

Primer extension 3' end

1211

AGGTGTTTTTGATGTTCTTGAAATGCC

Primer extension

1212

TTCGATAACAATTCCATTAGTGGACCC

Primer extension

1213

GATTTGCAGCATAAATAACGAGTTCCTG

Primer extension

1214

ATCTTCTAAAAAGATGGTACATATAGC

Primer extension

1215

ATCTATACGCAAAAGACATCACCTATC

Primer extension

1222

AAGTGATATTACCTTAAATAAGCGG

Primer extension 3' end

1224

AATCACTAGATTACACTGAGTAAGTG

Primer extension 3' end

1319

TAATTCTGAATCTCCAATACG

GSP*1 at 3' end of dnaG for 5' RACE

1320

TTTGAATATAGTCCTCTATTTCG

GSP*2 at 3' end of dnaG for 5' RACE

1321

TAACTAAATCTAATATATCAG

GSP*1 at 5' end of dnaG for 5' RACE

1322

TTTATTTCATCAATGACGGATTG

GSP*2 at 5' end of dnaG GSP2 for 5' RACE

731

GTAAGTCCATGG CATGGATAATATAAAGATAATTG

Forward serp1129 cloning primer with NcoI site

732

GATCAGGATCC GGTTGCTAAAAGAATGAAGG

Reverse serp1129 cloning primer with BamHI site

  1. *GSP = Gene specific primer
  2. Italics in primers 731 and 732 represent the Nco I and Bam HI restriction sites, respectively.