Figure 6From: Multiple controls affect arsenite oxidase gene expression in Herminiimonas arsenicoxydansAmino acids conservation between σ54 Enhancer Binding Proteins (EBP) and AoxR. Sequence alignment was performed with ClustalW. The conserved amino acids are presented with a blue background and the blue intensity reflects sequence similarities. Only the central binding domain is indicated. Region CI has remarkable similarity to the consensus glycine-rich flexible loop motif (Walker A - consensus motif GxxGxGK), and also contains hydrophobic residues. Region CII is hydrophobic. The region CIII is predicted to fold into two alpha helices separated by a turn. This region is involved in a specific interaction between the EBP and the Eσ54 required for open promoter complex formation via the GAFTGA motif. Region CIV is rich in glycine, negatively charged and contains a consensus sequence of 4 aliphatic residues followed by 2 negatively charged residues (Walker B - consensus motif TVFLDE); in contrast, CVI is positively charged and is rich in aromatic residues and proline. Region CV is found about 80 amino acids away from region CI, and has a consensus sequence QakLLRVLqe. Finally, region CVII has a core of eight highly conserved amino acids. Sequence informations of other genes were obtained from Colibri database (Institut Pasteur, Paris).Back to article page