Figure 5From: Identification of genes involved in the mutualistic colonization of the nematode Heterorhabditis bacteriophora by the bacterium Photorhabdus luminescensPolymyxin sensitivity of P. luminescens. TT01gfp and mutant strains were grown overnight in LB broth and inoculated into fresh LB without (black curve) or with (grey curves) 2.5 μg ml-1 polymyxin B (PB) added. The cells were grown for 24 h at 30°C and OD600 readings were taken every 15 mins. The growth curves of all of the strains were identical in the absence of added PB and therefore only a single representative curve (of TT01gfp) is shown. The growth curves of the strains growing in the presence of PB are labeled appropriately. Although the experiment was repeated 3 times only a representative growth curve of each mutant is presented.Back to article page