Rep. | Levels of spiking (CFU/g) | Amount of cellsb(CFU/rxn) | LAMP | PCR | |||
---|---|---|---|---|---|---|---|
 |  |  | Fluorescence-based | Turbidity-based | F3/B3 | toxR | |
 |  |  | Ct (min) | Mt (°C) | Tt (min) |  |  |
1 | 5.6 × 108 | 1.0 × 106 | 20.61 ± 2.04 | 82.16 ± 0.05 | 31.2 ± 2.97 | + | + |
 | 5.6 × 107 | 1.0 × 105 | 22.02 ± 2.04 | 81.36 ± 1.20 | 35.3 ± 1.13 | + | + |
 | 5.6 × 106 | 1.0 × 104 | 25.26 ± 0.56 | 81.87 ± 0.10 | 42.55 ± 2.2 | + | + |
 | 5.6 × 105 | 1.0 × 103 | 34.58 ± 2.25 | 82.45 ± 0.23 | 52.45 ± 2.75 | + | - |
 | 5.6 × 104 | 1.0 × 102 | - | - | - | - | - |
 | 5.6 × 103 | 10 | - | - | - | - | - |
2 | 1.7 × 108 | 3.1 × 105 | 21.78 ± 0.59 | 82.41 ± 0.11 | 29.4 ± 0.85 | + | + |
 | 1.7 × 107 | 3.1 × 104 | 23.68 ± 0.16 | 82.25 ± 0.10 | 33.25 ± 0.35 | + | + |
 | 1.7 × 106 | 3.1 × 103 | 29.08 ± 0.45 | 82.60 ± 0.34 | 40.4 ± 4.67 | + | - |
 | 1.7 × 105 | 3.1 × 102 | 31.77 ± 2.23 | 82.50 ± 0.18 | 47.7 ± 1.27 | - | - |
 | 1.7 × 104 | 31 | - | - | - | - | - |
 | 1.7 × 103 | 3.1 | - | - | - | - | - |
3 | 1.1 × 109 | 2.0 × 106 | 20.74 ± 0.03 | 82.48 ± 0.01 | 31.25 ± 4.02 | + | + |
 | 1.1 × 108 | 2.0 × 105 | 24.14 ± 0.24 | 82.37 ± 0.05 | 35.55 ± 3.73 | + | + |
 | 1.1 × 107 | 2.0 × 104 | 27.42 ± 0.60 | 82.48 ± 0.11 | 40.75 ± 3.88 | + | + |
 | 1.1 × 106 | 2.0 × 103 | 33.26 ± 2.84 | 82.50 ± 0.26 | 44.8 ± 0.7 | + | - |
 | 1.1 × 105 | 2.0 × 102 | 35.57 ± 1.73 | 82.65 ± 0.09 | 47.25 ± 0.35 | - | - |
 | 1.1 × 104 | 20 | - | - | - | - | - |