Table 4 Evaluation of the specificity and sensitivity of Mab 6B8 and 4C2 against H5 subtype influenza viruses.
Virus | Clade for H5N1 or Subtype | Absorbance reading in AC-ELISA (OD490)a | Detection Limit (HA unit) in dot ELISA | Detection Limit (HA unit) in dot ELISA (Rockeby) |
|---|---|---|---|---|
A/Hong Kong/156/97 | 0 | 1.323 | 0.25 | 1 |
A/Hong Kong/213/03 | 1 | 0.965 | 0.5 | 1.5 |
A/Vietnam/1203/04 | 1 | 1.235 | 0.25 | 1 |
A/Indonesia/CDC7/06 | 2.1.1 | 1.149 | 0.25 | 1.5 |
A/Indonesia/CDC594/06 | 2.1.2 | 1.326 | 0.125 | 1 |
A/Indonesia/CDC370/06 | 2.1.3 | 0.963 | 0.5 | 1.5 |
A/Indonesia/CDC523/06 | 2.1.3 | 1.234 | 0.25 | 1.5 |
A/Indonesia/CDC326/06 | 2.1.3 | 1.062 | 0.25 | 1 |
A/Indonesia/CDC669/06 | 2.1.3 | 1.085 | 0.5 | 1.5 |
A/turkey/Turkey1/05 | 2.2 | 1.247 | 0.25 | 0.5 |
A/barheaded goose/Qinghai/12/05 | 2.2 | 1.096 | 0.25 | 1 |
A/Nigeria/6e/07 | 2.2 | 0.954 | 0.5 | 1.5 |
A/Anhui/1/05 | 2.3 | 0.853 | 0.5 | 1.5 |
A/chicken/Nongkhai/NIAH400802/07 | 2.3 | 1.047 | 0.5 | 1 |
A/Vietnam/HN31242/07 | 2.3 | 1.247 | 0.5 | 1.5 |
A/goose/Guiyang/337/06 | 4 | 1.193 | 0.25 | 0.5 |
A/chicken/Shanxi/2/06 | 7 | 1.085 | 0.5 | 1 |
A/chicken/Henan/12/04 | 8 | 0.975 | 0.5 | 1.5 |
A/Puerto Rico/8/34 | H1N1 | 0.052 | - | 1 |
A/Singapore/TLL10/2009 | H1N1 | 0.046 | - | 1 |
A/Singapore/TLL54/2009 | H1N1 | 0.058 | - | 0.5 |
A/duck/Nanchang/4-184/2000 | H2N9 | 0.056 | - | 1 |
A/chicken/Singapore/02 | H3N2 | 0.061 | - | 1 |
A/Netherlands/219/03 | H7N7 | 0.059 | - | 1.5 |