Table 1 List of bacterial strains, plasmids and oligonucleotides
strain, plasmid or oligonucleotide | relevant characteristics or sequence | source or reference |
|---|---|---|
E. coli strains | ||
DH5α | F- thi-1 endA1 hsdR17(r- m-) supE44 ΔlacU169 (ϕ80lacZ ΔM15) recA1 gyrA96 relA1 | [32] |
Corynebacterium strains | ||
C. glutamicum ATCC 130302 | Â | ATCC [61] |
::dld | dld inactivation mutant of ATCC 13032 | This work |
C. efficiens DSM44547 | Â | DSM |
Plasmids | ||
pEKEx3 | SpecR; Ptac, lacIq | [24] |
pEKEx3-dld | pEKEx3 containing dld from C. glutamicum and an artificial ribosome binding site | this work |
pVWEx1 | KanR; Ptac, lacIq | [34] |
pVWEx1-dld | pEKEx3 containing dld from C. glutamicum and an artificial ribosome binding site | This work |
pK18mob | KanR; integration vector for C. glutamicum | [62] |
pK18mob-dld | pK18mob carrying an internal fragment of dld | This work |
Oligonucleotides | sequence | underlined nucleotide (position in NC003450) |
rbs-ndld | GTCGACAAGGAGATATAGAT A TGACGCAACCAGGACAGAC SalI | 955683 |
cdld | GGAGCTCT TAGGCCCAGTCCTTGTGC SacI | 957398 |
Ex-dld-fw | GCCCTGCAGG AAGGAGATATAGAT A TGACGCAACCAGGACAGAC SbfI | 955683 |
Ex-dld-bw | GCGGTACC T TAGGCCCAGTCCTTGTGC KpnI | 957398 |
dld1 | AATCATA TGAGACGCAACCAGGACAGACCACC NdeI | 955683 |
dld2 | AATGGATCCG GCCCAGTCCTTGTGCGGCGACGTGC BamHI | 957398 |
Cg-dld-SalI-N498 | AAGTCGACAGCCAGATTCCAGATTCGCAAGGGT SalI | 956907 |
Cg-dld-C1716-SalI | GGTCGACT TAGGCCCAGTCCTTGTGC SalI | 955683 |