Table 1 Oligonucleotide primers and probes for detection of S. pneumoniae, H. influenzae and N. meningitidis.

S. pneumoniae

Spn9802 F

5'-A GTC GTT CCA AGG TAA CAA GTC T-3'

3370-3392

Spn9802 R

5'-AC CAA CTC GAC CAC CTC TTT-3'

3525-3506

Spn9802 FAM

5'-^FAM-aTc AGa TTg CTg ATa AAa CgA-^BHQ1-'3

H. influenzae

Hi P6 F

5'-CCA GCT GCT AAA GTA TTA GTA GAA G-3'

302-326

Hi P6 R

5'-TTC ACC GTA AGA TAC TGT GCC-3'

477-457

Hi P6 JOE

5'-^JOE- CAg ATg CAg TTg AAg GTt Att tAG-^BHQ1-'3

N. meningitidis

5'-GCTGCGGTAGGTGGTTCAA-3'

617-635

ctrA R

5'-TTGTCGCGGATTTGCAACTA-3'

727-708

ctrA ROX

5'-^ROX-CATTGCCACGTGTCAGCTGCACAT- ^BHQ1-'3