Figure 6From: Virulence regulator AphB enhances toxR transcription in Vibrio choleraeThe influence of AphB on V. cholerae outer membrane composition, SDS resistance, and toxT expression. (A). Analysis of outer membrane preparations of V. cholerae derivatives. SDS-PAGE gel stained with Coomassie blue. OmpT and OmpU are indicated at the right. The minimum bactericidal concentration (MBC) of SDS is listed below the SDS-PAGE gel. (B). Wild type or aphB mutant containing a P toxT -luxCDABE reporter plasmid with or without pBAD-tcpPH were grown under the AKI condition. 0.01% arabinose was added to induce P BAD -tcpPH. Lux expression (blue bars) was measured and normalized against toxT expression in wild type. The results are the average of three experiments ± SD.Back to article page