Figure 2From: A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteinsProteolytic activity of P. brasiliensis protein extracts. Yeast cells were incubated in chemically defined MMcM medium with or without nitrogen sources (ammonium sulfate, asparagine and cystine) for 8 h. Protease activity was obtained by using azocasein assay. Activity was measured at 436 nm. A: Protease activity obtained in protein extracts of yeast cells incubated in MMcM medium. 1: without protease inhibitors; 2: with PMSF (1 mM); 3: with Pepstatin A (100 μM); 4: with EDTA (5 mM). B: Protease activity obtained in protein extracts of yeast cells incubated in MMcM medium without nitrogen sources. 1: without protease inhibitors; 2: with PMSF (1 mM); 3: with Pepstatin A (100 μM); 4: with EDTA (5 mM). Asterisk denotes values statistically different from control (P ≤ 0.05).Back to article page