Table 1 E. coli strains and plasmids used in the presented study
From: Genes regulated by the Escherichia coli SOS repressor LexA exhibit heterogenous expression
Bacterial strains | Relevant properties | Source/reference |
|---|---|---|
RW118 | thr-1 araD139 Δ(gpt-proA)62 lacY1 tsx-33 supE44 galK2 hisG4 rpsL31 xyl-5 mtl-1 argE thi-1 sulA211 | R. Woodgate |
RW464 | RW118 recA | R. Woodgate |
RW542 | RW118 lexA51 (Def) | R. Woodgate |
Plasmids | ||
pSC101 derivative | pSC101 low copy plasmid origin with promoterless GFPmut3 gene, Knr | 21 |
pSC300 | caa-gfp Knr | This study |
pSC301 | cna-gfp Knr | This study |
pSC302 | ce1a-gfp Knr | This study |
pSC303 | ce7a-gfp Knr | This study |
pSC304 | cma-gfp Knr | This study |
pColA-CA31 | caa cai cal | A. P. Pugsley |
pColN-284 | cna cni cnl | A. P. Pugsley |
pColE1-K53 | ce1a ce1i ce1l | A. P. Pugsley |
pColE7-K317 | ce7a ce7i ce7l | A. P. Pugsley |
pCHAP1 | cma | A. P. Pugsley |
pSC200 | lexA-gfp Knr | 21 |
pSC201 | recA-gfp Knr | 21 |
pSC202 | umuD-gfp Knr | 21 |
pSC203 | uvrA-gfp Knr | 21 |
pDsRed-Express2-N1 | DsRed-Expre ss2 reporter Knr | B. Glick |
pKCT3 | cka-gfp Apr Knr | 19 |
pKCT10 | cka-DsRed-Express2 Apr | This study |