Table 2 List of primers used in this study
Primer | DNA sequence* | Restriction site |
|---|---|---|
Expression | Â | Â |
   NMB0207(F) | CGCGGATCC ATGGGCATCAAAGTCGCCATC | Bam HI |
   NMB0207(R) | CGCGTCGAC TTATTTGAGCGGGCGCACTTC | Sal I |
Mutagenesis | Â | Â |
   NMB0207(R)FL | GAGAACTGTCATGCGTATTCC |  |
   NMB0207(F)FL | CCAAACCCAATGCCGCGAATG |  |
   gapA1_M1(IR) | GCGAGATCT GCAACAAACCGTC | Bgl II |
   gapA1_M2(IF) | GCGAGATCT GGTTTGTTCCTTTGTTGAGGG | Bgl II |
Complementation | Â | Â |
   pSAT-12iPCR(IF) | CGCAGATCT GATACCCCCGATGAC | Bgl II |
   pSAT-12iPCR(IR) | CGCAGATCT CATTTGTGTC TCCTTGG | Bgl II |
   gapA1_Comp(F)2 | CGCGGATCC ATGGGCATCAAAGTC | Bam HI |
   gapA1_Comp(R)2 | CGCGGATCC TTTGTTTGACGGTTTGTTG | Bam HI |