Table 1 Bacterial strains and plasmids
Strain or plasmid | Description | Source or reference |
|---|---|---|
Strains | Â | Â |
   E. coli |  |  |
JM109 | Cloning strain | Promega, Madison, WI |
TOP10F' | Cloning strain | Invitrogen, Carlsbad, CA |
BL21(DE3)pLysS | Expression strain | Invitrogen, Carlsbad, CA |
   N. meningitidis |  |  |
MC58 | wild-type serogroup B strain | [26] |
MC58ΔgapA-1 | gapA-1 deletion and replacement with kanamycin cassette | This study |
MC58ΔgapA-1 gapA-1Ect | MC58ΔgapA-1 complemented with an ectopic copy of gapA-1 | This study |
MC58ΔsiaD | siaD deletion and replacement with erythromycin cassette | C. Tang Imperial College |
MC58ΔsiaD ΔgapA-1 | siaD and gapA-1 deficient strain generated from MC58ΔsiaD using pSAT-8 | This study |
Plasmids | Â | Â |
pCRT7/NT-TOPO | Cloning vector encoding resistance to ampicillin | Invitrogen, Carlsbad, CA |
pDT-GapA1 | MC58 gapA-1 gene cloned in pCRT7-TOPO | This study |
pGEM-T Easy | Cloning vector encoding resistance to ampicillin | Promega, Madison, WI |
pSAT-6 | 3-kb fragment spanning the MC58 gapA-1 region cloned in pGEM-T Easy | This study |
pJMK30 | Source of kanamycin resistance cassette | [43] |
pSAT-8 | pSAT-6 containing the kanamycin resistance cassette in the same orientation as the deleted gapA-1 gene | This study |
pSAT-12 | Complementation vector containing cbbA and encoding resistance to erythromycin | [29] |
pSAT-14 | pSAT-12 containing gapA-1 in place of the deleted cbbA | This study |