Table 3 Bacterial strains and plasmids used in the present study
From: A rapid and simple method for constructing stable mutants of Acinetobacter baumannii
Strain or plasmid | Relevant feature(s) | Source or reference |
|---|---|---|
Strains | ||
Acinetobacter baumannii | ||
ATCC 17978 | Wild-type strain | Laboratory stock |
omp33::TOPO | Derived from ATCC 17978. omp33 mutant obtained by plasmid insertion. KanR, ZeoR | Present study |
Δomp33::Km | Derived from ATCC 17978. omp33 mutant obtained by gene replacement. KanR | Present study |
ΔoxyR::Km | Derived from ATCC 17978. oxyR mutant obtained by gene replacement. KanR | Present study |
ΔsoxR::Km | Derived from ATCC 17978. soxR mutant obtained by gene replacement. KanR | Present study |
ΔoxyR::Km-omp33::TOPO | Derived from ΔoxyR::Km. oxyR omp33 double mutant. KanR, ZeoR | Present study |
ΔsoxR::Km-omp33::TOPO | Derived from ΔsoxR::Km. soxR omp33 double mutant. KanR, ZeoR | Present study |
Escherichia coli | ||
TG1 | supE thi-1 Δ(lac-pro AB) Δ(mcr B-hsd SM)5(rK- mK-) [F' traD 36 pro AB lac IqZΔM15] | Laboratory stock |
Plasmids | ||
pCR-BluntII-TOPO | Suicide plasmid for A. baumannii. KanR, ZeoR | Invitrogen |
pTOPO33int | pCR-BluntII-TOPO containing a 387-pb internal fragment of the omp33 gene. KanR, ZeoR | Present study |
pET-RA | A. baumannii replication origin. CTX-M14 β-lactamase gene promoter. RifR | Present study |
pET-RA-OMP33 | pET-RA containing the omp33 gene without its promoter region. RifR | Present study |