Table 1 Bacterial strains and plasmids

Escherichia coli

DH5α

F^- ϕ80ΔlacZM15 Δ(lacZYAargF) U169 deoP recA1 endA1 hsdR17 (r_k^- m_k^-)

Gibco BRL

TOP10F'

F' {lacIq Tn10(TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG

Invitrogen

Salmonella enterica serovar Typhimurium

SL1344

xyl hisG rpsL; virulent; Sm^R

[30]

CMPG5602

SL1344 ΔluxS - deletion of the entire luxS CDS

[10]

CMPG5702

SL1344 luxS::Km^R

[14]

CMPG5630

SL1344 ΔluxS2 - deletion of the 3' end of luxS CDS

This study

CMPG5628

SL1344 Δhfq

This study

JVS-01028

SL1344 ΔrpoE::Cm^R

[34]

CMPG5643

SL1344 ΔompA::Km^R

Phage lysate of J. Vogel

CMPG5648

SL1344 ΔlamB

This study

Plasmids

pJV300

pZE12-luc based plasmid; P_LlacO-rrnB terminator; short nonsense transcript; control plasmid; Amp^R

[35]

pJV871.14

pZE12-luc based plasmid; LT2 MicA overexpression construct; MicA transcription driven from constitutive P_LlacO, starting precisely at G₊₁; Amp^R

[36]

pJV853.1

pZE12-luc based plasmid; LT2 anti-MicA expression construct; anti-MicA transcription driven from constitutive P_LlacO; Amp^R

J. Vogel,

unpublished data

pKD3

Template for mutant construction; carries

chloramphenicol-resistance cassette; oriRγ origin; Amp^R

[31]

pKD46

P_araB-γ-β-exo; temperature-sensitive lambda-red

recombinase expression plasmid; oriR101 origin; Amp^R

[31]

pCP20

Temperature-sensitive FLP recombinase

expression plasmid; oriR101 origin; Amp^R, Cm^R

[31]

pFAJ1708

Derivative of RK-2; Amp^R; Tc^R; contains nptII promoter of pUC18-2

[32]

pCMPG5685

pFAJ1708 OmpA complementation construct

This study

pCMPG5687

pFAJ1708 LamB complementation construct

This study

pCMPG5638

pCS26-Pac plasmid carrying a transcriptional reporter fusion between the promoter of the lsrACDBFGE operon and luxCDABE

[10]