Strain or plasmid | Relevant genotype and/or description | Source or reference |
---|---|---|
C. salexigens strains | Â | Â |
   DSM 3043T | Wild type | [19] |
   CHR61 | Spontaneous Rfr mutant of C. salexigens DSM 3043 | [21] |
   CHR95 | CHR61 ΔeupRmntR::Tn1732; Rfr Kmr | This study |
   CHR161 | CHR61 mntR::Ω; Rfr Smr Spcr | This study |
   CHR183 | CHR61 eupR::Ω; Rfr Gnr | This study |
E. coli strain | Â | Â |
   DH5α | supE44 Δ(lac)U169 ϕ80dlacZ ΔM15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1; host for DNA manipulations | [65] |
Plasmids | Â | Â |
   pKS(-) | Cloning vector; Apr | Stratagene |
   pHP45Ω | pBR322 derivative carrying the Ω cassette; Apr Smr Spr | [50] |
   pHP45Ωaac | pBR322 derivative carrying the Ωaac cassette; Apr Gmr Gnr | [51] |
   pRK600 | Helper plasmid; Cmr tra | [66] |
   pJQ200-SK | Suicide vector; Gmr mob sac | [52] |
   pSUP102-Gm::Tn1732 | Mutagenesis plasmid carrying Tn1732; Cmr Kmr Gmr | |
   pRR1 | pKS derivative carrying a 20.8-kb sac I fragment from CHR95 including Tn1732; Apr Kmr | This study |
   pMntREupR | 3-kb Xba I-Apa I fragment from C. salexigens genome (containing orf1, eupR, mntR, orf4) cloned into pKS; Apr | This study |
   pHpaIMntr | pMntREupR derivative containing a Hpa I recognition site within mntR; Apr | This study |
   pHindIIIEupR | pMntREupR derivative containing a Hin dIII recognition site within eupR; Apr | This study |
   PΩMntR | pHpaIMntr derivative with Ω cassette within mntR; Apr Smr Spr | This study |
   pΩEupR | pHindIIIEupR derivative with Ωaac cassette within eupR; Apr Gnr Gmr | This study |
   pJQMntR | 5-kb Xba I-Apa I fragment from pΩMntR (containing orf1, eupR, mntR::Ω, orf4) cloned into pJQ200-SK; Gmr Smr Spr | This study |
   pJQEupR | 5-kb Xba I-Apa I fragment from pΩEupR (containing orf1, eupR::Ωaac, mntR, orf4) cloned into pJQ200-SK; Gmr Gnr | This study |