Figure 3

Enzymatic activities of PhaC and PhaZ during growth of P. putida U on octanoate. P. putida U was grown on 15 mM octanoate in nitrogen limited medium (0.2 NE2). Culture aliquots were harvested, resuspended to 1 mg total protein/ml and lysed by three passages through a French pressure cell and analyzed for non-PHA biomass (x, right scale), accumulation of mcl-PHA relative to the total cell dry weight (cdw) (filled circle, right scale), activities of PhaC (open square, left scale) and PhaZ (open triangle, left scale). Data represent the average of two measurements.