Figure 5From: Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)Purification of AatAF after expression in E. coli BL21. The internal part of aatA encoding the passenger domain of AatA was cloned into pET32a(+) leading to the expression of the 64-kDa fusion protein AatAF. BL21 cells were incubated in LB at 37°C without (lane 1) or with (lane 3) addition of IPTG. Proteins of total extracts (lane 1 and 3) and of eluates of the purified AatAF (lane 4) were separated on an SDS-PAGE and stained with coomassie. TE: total extract, -: without IPTG, +: with IPTG; M: protein marker.Back to article page