Table 2 Gene expression profiles for phototrophic (light) vs. chemotrophic (dark) growth.
From: Energy metabolism of Heliobacterium modesticaldum during phototrophic and chemotrophic growth
Gene name | ΔCTa(light) | ΔCTa(dark) | ΔΔCTb | Relative gene expression level (light/dark)c |
|---|---|---|---|---|
Genes for carbon metabolism | ||||
pfkA (6-phosphofructokinase) | 15.0 ± 0.1 | 22.0 ± 0.1 | 7.0 ± 0.2 | 128 |
pykA (pyruvate kinase) | 13.5 ± 0.1 | 19.5 ± 0.1 | 6.0 ± 0.2 | 64 |
porA (pyruvate:Fd oxidoreductase) | 13.7 ± 0.1 | 11.6 ± 0.0 | -2.1 ± 0.1 | 0.2 |
fdxR (Fd-NADP+ oxidoreductase) | 14.7 ± 0.1 | 15.2 ± 0.1 | 0.5 ± 0.2 | 1.4 |
Ferredoxin | 13.4 ± 0.1 | 13.2 ± 0.1 | -0.2 ± 0.2 | 1 |
pshB (ferredoxin) | 14.0 ± 0.1 | 14.3 ± 0.1 | 0.3 ± 0.2 | 1 |
ackA (acetate kinase) | 10.6 ± 0.1 | 12.2 ± 0.1 | 1.6 ± 0.2 | 3 |
acsA (acetyl-CoA synthase) | 15.5 ± 0.1 | 21.0 ± 0.1 | 5.5 ± 0.2 | 45 |
ppdK (pyruvate phosphate dikinase) | 13.4 ± 0.1 | 17.4 ± 0.1 | 4.0 ± 0.2 | 16 |
pckA (PEP carboxykinase) | 14.1 ± 0.1 | 17.2 ± 0.1 | 3.1 ± 0.2 | 8 |
mdh (malate dehydrogenase) | 14.5 ± 0.1 | 14.6 ± 0.1 | 0.1 ± 0.2 | 1 |
Genes for pigment biosynthesis | ||||
bchY | 13.1 ± 0.1 | 15.7 ± 0.0 | 2.6 ± 0.1 | 6 |
bchB | 14.0 ± 0.0 | 18.0 ± 0.1 | 4.0 ± 0.1 | 16 |
bchE | 13.2 ± 0.1 | 15.0 ± 0.1 | 1.8 ± 0.2 | 4 |
bchG | 12.9 ± 0.1 | 13.9 ± 0.1 | 1.0 ± 0.2 | 2 |
Genes for nitrogen assimilation and hydrogen production | ||||
nifK (Fe/Mo nitrogenase, β subunit) | 13.0 ± 0.0 | 21.5 ± 0.1 | 8.5 ± 0.1 | 365 |
nifD (Fe/Mo nitrogenase, α subunit) | 13.7 ± 0.0 | 21.4 ± 0.1 | 7.7 ± 0.1 | 197 |
hupS ([NiFe]-hydrogenase small subunit) | 13.3 ± 0.1 | 18.4 ± 0.1 | 5.1 ± 0.2 | 34 |
hupL ([NiFe]-hydrogenase large subunit) | 12.7 ± 0.1 | 18.3 ± 0.1 | 5.6 ± 0.2 | 49 |
hymD (Fe only hydrogenase, Hymd subunit) | 13.4 ± 0.1 | 18.7 ± 0.1 | 5.3 ± 0.2 | 40 |
nuoE | 14.3 ± 0.2 | 19.7 ± 0.1 | 5.4 ± 0.3 | 43 |
nuoF | 12.9 ± 0.2 | 18.6 ± 0.1 | 5.7 ± 0.3 | 51 |
nuoG | 12.9 ± 0.1 | 18.6 ± 0.1 | 5.7 ± 0.2 | 51 |