In vivo bioluminescence imaging and histopathopathologic analysis reveal distinct roles for resident and recruited immune effector cells in defense against invasive aspergillosis

Table 1 Comparison between the lesion profiles in the different immunosuppressive conditions.

		Lesions			Recruted Inflammatory Cells		Fungi
		Proportion of inflammatory infiltrate surface	Major Localisation	Necrosis	Neutrophiles	Macrophages	Conidia	Hyphae
Chlodrolip	Early 1 day PI	1.8 ± 1.0%	Random distribution	-	++	+/-	++	-
	Late 8 days PI	2.9 ± 1.7%	Perivascular Peribronchiolar	-	-	+	+	-
Cortisone Acetate
	Early 1 day PI	3.8 ± 2.0%	Alveoli	++	++	+/-	++	+/-
	Late 3 days PI	11.2 ± 1.9%	Bronchi Bronchioles	+++	+++	++	++	+++
RB6-8C5
	Early 1 day PI	1.9 ± 0.5%	Random distribution	+/-	-	+/-	+	-
	Late 3 days PI	18.9 ± 2.8%	Bronchi Bronchioles	+++	-	+++	++	+++
Cyclophosphamide
	Early 1 day PI	No infiltrate	Bronchioles	+/-	+/-	-	+/-	-
	Late 3 days PI	No infiltrate	All structures	++++	-	-	+	++++

A group of five mice was studied at each time point, for each condition. The lesions were very similar between the different animals in a same group (PI: post-infection). A group of three mice was studied for the measurement of the surface of inflammatory cell infiltrates in tissue sections (morphometric analysis, 22 to 40 microscopic fields covering an entire lung section for each animal).
- none, +/- minimal, + mild, ++ moderate, +++ marked, ++++ severe

ISSN: 1471-2180