Figure 1From: In vivo bioluminescence imaging and histopathopathologic analysis reveal distinct roles for resident and recruited immune effector cells in defense against invasive aspergillosisClodrolip treated mice are not susceptible to A. fumigatus intranasal infection. In each experiment, groups of 5 mice were treated either with cortisone acetate, cyclophosphamide, RB6-8C5 antibody, or clodrolip prior to intranasal infection with 2 × 106 conidia of the luminescent A. fumigatus strain C3. Untreated infected mice are designated as immnocompetent (IC). Weight loss and survival were monitored for 8 days (A and B). (C): Time response study of luminescence emission from chest region 10 min after intraperitoneal injection of D-luciferin. Light emission from live animals was recorded for 5 min. Each point represents the average from 3 independent experiments of the total photon flux measured from a defined thoracic region from each individual animal of the respective cohort (5 mice). (D): Light emission from the lung of a dead animal immunosuppressed with cortisone acetate following direct injection of D-luciferin. A total photon flux/second of 3.744 × 106 has been measured using the living image software 3.1 after 1 min exposure.Back to article page