Figure 1From: Functional dissection of translocon proteins of the Salmonella Pathogenicity Island 2-encoded type III secretion systemBioinformatic analyses of SPI2 translocon protein SseB and characteristics of deletion variants of SseB. A) Using the program TMpred, putative transmembrane (TM) domains of the translocon protein SseB was predicted. o-i indicate the strongly preferred model, with N-terminus outside (aa 38-57), i-o indicates the alternative model. B) Using the program COILS, coiled-coil regions in SseB were predicted. As output option the default parameters were selected that gave residue number, residue type and the frame and coiled-coil forming probability obtained in scanning windows of 14, 21 and 28 residues (as described on the Swiss EMBnet homepage). The region spanning aa 58-90 was considered as coiled-coil domain. C) Schematic representation of the amino acid sequence of wild-type SseB and positions of deletions analyzed in this study. The predicted TM domain, coiled-coil region, as well as the chaperone-binding site [10] are indicated. The deleted regions within sseB variants are indicated by arrows and C- or N-terminal truncations are indicated by vertical red lines.Back to article page