Figure 3

SDS-PAGE and immunoblotting of purified whey proteins. A: lanes 1, 2 and 3 correspond, respectively, to the pooled protein not retained by Heparin-Sepharose (fSC), the pooled fractions eluted from Heparin-Sepharose with approximately 0.7 M NaCl (Lf) and commercial lactoferrin stained with coomassie blue; lanes 4, 5 and 6 correspond to the same samples revealed by their reaction with Lf antibodies. B: pooled fractions not retained by Heparin-Sepharose (fSC) stained with coomasie blue (lane 1) and immunostained by reaction with SC antibodies (lane 2).C: P3-S stained with coomasie blue (lane 1) and immunostained by reaction with a lactoalbumin antibodies (lane 2).