Figure 3
From: MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis
Autoradiograph showing the outcome of the MTR kinase assay. The assay was carried out for 90 min at 37°C (see Materials and Methods). Lane 1 corresponds to [•32P]ATP as standard; lanes 2, 4 and 6: no MTR was added for the reactions with cell-free extracts of wild type, BFS1850 and BFS1850 grown with IPTG, respectively; lanes 3, 5 and 7: reaction performed in the presence of MTR and cell-free extracts of wild type, BFS1850 and BFS1850 grown with IPTG, respectively.