Indole-3-acetic-acid and ACC deaminase producing Leclercia adecarboxylata MO1 improves Solanum lycopersicum L. growth and salinity stress tolerance by endogenous secondary metabolites regulation

Background The utilization of plant growth-promoting microbes is an environment friendly strategy to counteract stressful condition and encourage plants tolerance. In this regards, the current study was designed to isolate ACC deaminase and indole-3-acetic acid (IAA) producing halotolerant bacteria to promote tomato (Solanum lycopersicum L.) growth and tolerance against salinity stress. Results The selected bacterial isolate MO1 was identified as Leclercia adecarboxylata and IAA quantification results revealed that MO1 produced significant amount of IAA (9.815 ± 0.6293 μg mL− 1). The MO1 showed the presence of ACC (1-Aminocyclopropane-1-Carboxylate) deaminase responsible acdS gene and tolerance against salinity stress. A plant microbe interaction experiment using tomato (Solanum lycopersicum L.) with glycine betaine (GB) as a positive control was carried out to investigate the positive role MO1 in improving plant growth and stress tolerance. The results indicated that MO1 inoculation and GB application significantly increased growth attributes under normal as well as saline condition (120 mM NaCl). The MO1 inoculation and GB treatment approach conferred good protection against salinity stress by significantly improving glucose by 17.57 and 18.76%, sucrose by 34.2 and 12.49%, fructose by 19.9 and 10.9%, citric acid by 47.48 and 34.57%, malic acid by 52.19 and 28.38%, serine by 43.78 and 69.42%, glycine by 14.48 and 22.76%, methionine by 100 and 124.99%, threonine by 70 and 63.08%, and proline by 36.92 and 48.38%, respectively, while under normal conditions MO1 inoculation and GB treatment also enhanced glucose by 19.83 and 13.19%, sucrose by 23.43 and 15.75%, fructose by 15.79 and 8.18%, citric acid by 43.26 and 33.14%, malic acid by 36.18 and 14.48%, serine by 46.5 and 48.55%, glycine by 19.85 and 29.77%, methionine by 22.22 and 38.89%, threonine by 21.95 and 17.07%, and proline by 29.61 and 34.68% compared to levels in non-treated plants, respectively. In addition, the endogenous abscisic acid (ABA) level was noticeably lower in MO1-inoculated (30.28 and 30.04%) and GB-treated plants (45 and 35.35%) compared to their corresponding control plants under normal condition as well as salinity stress, respectively. Conclusion The current findings suggest that the IAA- and ACC-deaminase-producing abilities MO1 can improve plants tolerance to salinity stress. Electronic supplementary material The online version of this article (10.1186/s12866-019-1450-6) contains supplementary material, which is available to authorized users.


Background
The unpredictable climatic changes resulting from the rapidly growing population and anthropogenic activities bring various unwanted environmental stresses that negatively affect agriculture productivity [1]. Environmental changes have worsened the rigorousness of various stressors, which negatively influence the agriculture production at the present Anthropocene Era. Meanwhile, food security is needed to maintain for the growing global population by increasing crop production in sustainable and environment friendly way. Among the various environmental stresses caused by climate change, salinity stress represents a major threat to agriculture productivity [2,3]. Soil salinity is reported to negatively affect more than 77 million hectares, substantially reducing major crops by more than 50% [4]. Currently, around 20% of cultivated land and 50% of irrigated land is effected by salinity stress [5]. Salinity stress has been shown to negatively affect plant growth agronomically and biochemically by reducing nearly all growth attributes, carbon acclimatization, nitrogen metabolism, and grain yield [6][7][8]. Salinity has three possible effects on plants, namely interfering with essential nutrient uptake, imparting toxicity because of the higher absorbance of Na + and Cl − , and lowering the water potential [9][10][11]. During osmotic and ionic stress triggered in plants by salt stress, the generation of active oxygen species, including superoxide (O 2 -), hydroxyl radicals (· OH), hydrogen peroxide (H 2 O 2 ), and singlet oxygen (1O 2 ), is thought to play an important role in inhibiting plant growth; furthermore, the active oxygen species need to be scavenged for the maintenance of normal growth [12,13].
Salt stress is a serious concern in the areas with optimum climate for tomato cultivation [14]. According to USDA, tomato is moderately sensitive to salt stress [15,16]. Salinity stress effect tomato growth via morphological, physiological and metabolic changes. However, many researchers reported high level of carbohydrates, amino acids, organic acids and total soluble solids in tomato plants grown in high saline conditions [17,18].
A variety of methods have been used to improve crop tolerance against salinity stress, including traditional and genetic engineering methods, but one of the most promising methods is the utilization of plant growth-promoting microorganisms to promote stress tolerance and growth [19][20][21]. Many researchers have confirmed the effectiveness of the utilization of phytobeneficial microorganisms for stress mitigation and plant growth promotion [22][23][24]. Among plant growth-promoting microorganisms, plant growth-promoting rhizospheric bacteria (PGPR) are of great importance because of their direct association with plant roots [25]. PGPR are able to colonize roots, facilitate growth, and mitigate various stresses either directly by producing phytohormones (Gibberellins (GAs), Abscisic acid (ABA), Indole-3-acetic-acid (IAA)), improving nutrient uptake, producing siderophores, and solubilizing minerals or indirectly by decreasing plant pathogens [25,26]. Various PGPR have been found to mitigate salt stress, improve growth, and enhance tolerance in various crop plants [27][28][29][30].
ACC deaminase and IAA producing bacteria assist plant growth and can effectively protect plants against various environmental stresses, including salinity stress [31]. Rhizobacteria use tryptophan and other small molecules in root exudates and convert them into indole-3-acetic acid, which is utilized by the plant roots and resulted in the activation of plant's endogenous auxin signaling pathway, which contribute in the growth promotion and proliferation of plant cells [32]. IAA accumulation in plants induces the transcription of ACC synthase genes, which increases the ACC concentration, leading to the production of ethylene. PGPR containing ACC deaminase may break down some of the excess ACC and lower plant ethylene levels during the advent of environmental stress [33].
Within this context, this study was designed to isolate ACC deaminase and indole-3-acetic acid (IAA) producing halotolerant PGPR because the survival and adaptation of PGPR to stressful environments are important to understand and confirm the survival of bacteria in real high-saline habitats. Moreover, the current study focused on investigating the various physio-chemical responses of tomato (Solanum lycopersicum L.) with the intention to explain the regulatory networks involved in osmotic stress tolerance and enhanced salinity stress tolerance following inoculation with ACC deaminase-and IAA-producing halotolerant PGPR.

Isolation, selection and identification of Leclercia adecarboxylata MO1
The soil samples were collected from the rhizosphere of tomato (Solanum lycopersicum L.) plant at vegetative growth stage. The collected soil samples (10 g) were transferred to a 250 mL flask containing 100 mL Amies solution and then serially diluted to 1 × 10 − 4 . Then, a 0.1 mL suspension was spread on tryptic soy agar plates (TSA; Merck Co., Germany) and incubated at 28°C for 24 h. The incubated plates were examined every 6 h to assess bacterial growth, and the newly appeared bacteria were re-streaked on new plates to get obtain colonies. Among total 36 isolated strains, Leclercia adecarboxylata MO1 was successfully isolated and selected on its IAA-producing capability and its ACC deaminase synthesis gene based on the Salkowski test and PCR results (Fig. 1). In addition, MO1 was grown under controlled conditions and under treatments with 120, 250, and 500 mM NaCl to examine its growth. The results showed that MO1 had maximal growth with a cell density of 3.47 ± 0.027 at OD 600 in medium supplemented with 120 mM NaCl compared to that under control conditions, with a cell density of 3.21 ± 0.031. However, the higher concentrations (250 and 500 mM NaCl) negatively affected the growth of MO1, resulting in cell densities of 2.23 ± 0.064 and 1.09 ± 0.097 at OD 600 , respectively (Fig. 1).
Furthermore, MO1 was identified by 16S rRNA sequencing. An NCBI (http://www.ncbi.nlm.nih.gov/) BLAST search of the 16S rRNA sequence revealed that MO1 is closely related to the genus Leclercia. To further confirm this finding, a detailed phylogenetic analysis (MEGA 6.0) was performed, closely related sequences were aligned, and a neighbor-joining tree was constructed using MEGA 6.0 (Fig. 2). The MO1 showed high sequence homology and formed a subclade with Leclercia adecarboxylata. Based on these results, MO1 was identified as L. adecarboxylata, and its 16S rRNA sequence was submitted to the NCBI gene bank under the accession number KP676112.

IAA production by MO1
The presence of IAA produced by MO1 in the inoculated cultural broth was initially detected with the Salkowski reagent and was further quantified by GC/MS (Fig. 1). The quantification results revealed that a significant amount of IAA (9.815 ± 0.6293 μg/mL) was produced by MO1 (Fig. 1).

Effects of MO1 inoculation on plant growth and stress mitigation
The phytobeneficial and salinity stress-mitigating efficiency of MO1 were assessed by a plant microbe-interaction experiment using GB as positive and water as negative control. The results showed the positive correlation of MO1 with plant. The MO1 inoculation and GB treatment produced a fundamentally beneficial response under normal and stress conditions, while the NaCl treatment resulted in a hindered and lower growth potential (Fig. 3).
MO1 inoculation and GB treatment significantly improved all growth attributes, including shoot length, with 22.09 and 18% increases; root length, with 16.3 and 12.5% increases; shoot weight, with 28.01 and 27.22% increases; root weight, with 51.15 and 47.33% increases; and stem diameter, with 15.39 and 6.44% increases, respectively (Table 1).
Moreover, salinity stress decreased plant growth attributes, while the MO1 inoculation and GB treatment significantly protected the plant against salinity stress (  Table 1).

Influence of MO1 inoculation and GB treatment on chlorophyll fluorescence
The regular exposure of plants to salinity stress suppresses and negatively regulates the chlorophyll contents. In the current study, MO1 inoculation and GB treatment significantly improved the chlorophyll fluorescence (Fv/Fm) of plants as compared to control (Fig. 4). Under normal condition, MO1 inoculation and GB treatment resulted in a higher chlorophyll fluorescence, with 15.03 and 14.39% increases, respectively, compared to that in the control (Fig.  4). Under salinity stress, a similar trend of improved chlorophyll fluorescence following MO1 inoculation and GB treatment was observed. A higher chlorophyll fluorescence was noted in MO1-inoculated and GB-treated plants, with 54.11 and 50.82% increases, respectively, compared to non-treated plants (Fig. 4).

Sugar synthesis in response to MO1 inoculation and GB treatment during salinity stress
In current study, the MO1 and GB treatments enhanced the glucose, sucrose, and fructose levels in plants grown Moreover, under saline conditions, glucose increased by 18.76 and 17.57% in GB-treated and MO1-inoculated plants, respectively, compared with the levels in non-treated plants (Fig. 5). The sucrose and fructose levels significantly increased following MO1 inoculation by 34.2 and 19.9%, respectively, and following GB treatment by 12.49 and 10.9%, respectively, compared to the levels in non-treated plants grown under salinity stress (Fig. 5).

Organic acid regulation by MO1 inoculation and GB treatment under salinity stress
Organic acid contents (citric acid and malic acid) were significantly modulated following MO1 inoculation and GB treatment under normal and salinity stress conditions (Fig. 6). The results revealed significantly increased amounts of citric acid (43.26 and 33.14%) in MO1-inoculated and GB-treated plants, respectively, under control conditions (Fig. 6). Malic acid significantly increased by 36.18% following MO1 inoculation and by 14.48% following GB treatment compared to non-treated plants (Fig. 6).
A similar trend of differentially regulated citric and malic acid was found under salinity stress. The MO1 and GB treatments significantly increased the organic acid contents of plants under salinity stress (Fig. 6). The results revealed that the MO1 and GB treatments significantly increased the citric acid level, with 47.48 and 34.57% increases, respectively, compared to that in non-treated   (Fig. 6). Moreover, a significant increase in malic acid of 52.19% was observed in MO1-inoculated plants followed by a 28.38% increase with GB treatment compared with that in non-treated plants (Fig. 6).

Endogenous ABA modulation by MO1 and GB under stress
Stress-responsive endogenous ABA was notably modulated in MO1-inoculated and GB-treated plants (Fig. 8).
The results indicated that under control conditions, GB treatment significantly decreased the endogenous ABA level by 45% and that MO1 inoculation decreased it by 30.28% compared to the level in non-treated plants (Fig.  8).
The salinity stress exposure increased the endogenous ABA level in plants, while the MO1 inoculation and GB treatment significantly reduced the endogenous ABA level by 30.04 and 35.35%, respectively, compared to non-treated plants (Fig. 8).

Discussion
The utilization of plant growth-promoting microbes for stress tolerance is an ideal and eco-friendly strategy [29]. To date, several studies have been carried out, in which PGPR were utilized for their potent role in salinity stress mitigation [27,[34][35][36][37]. Thus, the current study was intended to isolate an halotolerant IAA-producing bacteria and assess its potential to promote tomato (Solanum lycopersicum L.) growth and tolerance against salinity stress, and the selected isolate was identified as L. adecarboxylata MO1 (Figs. 1 and 2). L. adecarboxylata, formerly known as Escherichia adecarboxylata, belongs to the Enterobacteriaceae family [38]. Because of electrophoretic and nucleic differences, the E. adecarboxylata was separated from the Enterobacter agglomerans and reclassified as L. adecarboxylata [39]. L. adecarboxylata is widely distributed in nature and has been isolated from various sources, including seeds, Fig. 4 Effects of L. adecarboxylata MO1 inoculation and GB treatment on the chlorophyll fluorescence (Fv/Fm) of plants under normal and salinity stress conditions. Bars with different letters indicate significant differences (P ≤ 0.05) among treatments based on DMRT. Each value represents the mean ± SD of six replicates from each of three independent experiments plants, rhizosphere, food, water, and various other environmental sources [38][39][40][41][42].
L. adecarboxylata is metabolically diverse and can produce phytohormones, synthesize extra-cellular enzymes, degrade hydrocarbons, and solubilize minerals [40,[43][44][45]. Such traits have been reported to improve plant growth and mitigate various stresses [46]. Enzymes include ACC (1-aminocyclopropane-1-carboxylic acid) daeminase are related with free living rhizobacteria, which play an important role in facilitating plant growth [47]. In current study L. adecarboxylata MO1 showed the presence of genes responsible for the deamination of ACC (Fig. 1). ACC, the immediate precursor for ethylene synthesis in plants is exuded from roots of plants, which is metabolized by bacteria having the potential to produce ACC deaminase [48]. ACC deaminase catalyzes the conversion of ACC to ammonia and α-ketobutyrate [49]. This conversion lower the ethylene level in plants and resulted in plant growth promotion. Moreover, there is much less ethylene in the presence of ACC deaminase and consequent ethylene feedback inhibition of IAA signal transduction, so that bacteria IAA can continue to promote the growth and increase ACC synthase transcription. This IAA and ACC deaminase cross-talking resulted in lowering the ethylene level and revealed that ACC deaminase facilitate the plant growth by IAA [47]. The IAA-producing potential of L. adecarboxylata MO1 (Fig. 1) is in line with that reported by Shahzad et al. [40]. Moreover, the growth-promoting of capability of L. adecarboxylata has been widely reported [50]; however, the role of L. adecarboxylata in stress mitigation is not well understood, making the proper investigation of its potent role in mitigating stress and improving stress tolerance necessary. The current study is the first report on the salinity stress mitigation potential of L. adecarboxylata.
In our study, salinity stress caused a drastic decrease in the chlorophyll fluorescence (Fv/Fm) of plants. However, the MO1 inoculation and GB treatment increased the chlorophyll fluorescence (Fig. 4). The increase in chlorophyll fluorescence under normal and stress conditions following L. adecarboxylata inoculation and GB treatment might be linked to the enhanced pigment synthesis [51]. Moreover, during chlorophyll synthesis, plants fix the carbon dioxide into sugar by using sunlight [52]. Sugar (glucose, sucrose, and fructose) is an important source of energy to stimulate plant growth and adecarboxylata MO1 and GB in salinity stress (Fig. 5). Under saline conditions, the thylakoid membranes are damaged, which results in reduced photosynthetic efficiency and less soluble sugar production [51].
Phytobeneficial microorganisms can stimulate organic acid metabolism in plants under stress conditions [55,56]. Organic acids play an important role as a solute in osmatic adjustment and excess cation balance under salinity stress [55]. In the current study, the MO1 Fig. 6 Organic acid regulation resulting from L. adecarboxylata MO1 inoculation and GB treatment under normal and saline conditions. Bars with different letters indicate significant differences (P ≤ 0.05) among treatments based on DMRT. Each value represents the mean ± SD of six replicates from each of three independent experiments inoculation and GB treatment significantly increased organic acids under normal as well as under salinity stress conditions (Fig. 6). Similar results of increases in organic acids following inoculation with plant growth-promoting bacteria under osmotic stress have been reported [55,57]. Organic acids also help plants cope with nutrient deficiencies and stress tolerance because they have the potent ability to displace phosphorus from insoluble complexes and make it available for uptake by plants [58]. Moreover, organic acids have also been reported to be involved in plant microbe interactions at the root/soil interface, as organic acids secreted by roots are known to act in chemical signaling for quorum sensing, exopolysaccharide secretion, and biofilm formation during rhizosphere colonization [59,60].
Amino acids are also used as either precursors or intermediates for important metabolites responsible for mitigating various biotic and abiotic stresses [61,62]. In addition, plant growth-promoting microbes also secrete amino acids in soil, and plants absorb them through their roots via important mechanisms [63,65]. The increased levels of amino acids in MO1-inoculated and GB-treated plants under normal and salinity stress conditions (Fig. 7) reflected their role in strengthening plant tolerance against salinity stress by various mechanisms [24].
In the current study, the endogenous ABA level was significantly reduced by MO1 inoculation and GB treatment (Fig. 8). The decrease in the ABA level revealed positive correlation between MO1 and GB and plant Fig. 7 Amino acid regulation by L. adecarboxylata MO1 and GB treatment in plants under normal and saline conditions. Bars with different letters indicate significant differences (P ≤ 0.05) among treatments based on DMRT. Each value represents the mean ± SD of six replicates from each of three independent experiments growth and stress tolerance. As ABA is involved in stress signaling, its biosynthesis can be affected during plant growth-promoting microbe interactions [24,64]. Several studies have reported increases in ABA under salinity and osmotic stresses. However, many plant growth-promoting microbes have been reported to reduce ABA accumulation under stress conditions [24,37,65]. However, some microorganisms have also been reported to increase the endogenous ABA level, but the effect depends on the various classes of microorganisms [66]. The reduction of ABA accumulation in MO1-inoculated plants is correlated with its IAA-producing capability, resulting in improved plant growth and improved water relation [67], suggesting an active role of PGPRB in stress resistance.

Conclusion
Salinity stress is one the major factors that hinder agriculture productivity, and the development of stress-resistant varieties via breeding and genetic engineering is a lengthy and expensive process. However, the utilization of plant growth-promoting microbes to alleviate stress is a more cost-effective and environmentally friendly approach. Delivery of ACC-deaminase and IAA via PGPR mostly affected ethylene and ABA-dependent signaling in positive way which facilitate plant growth and mitigate stressful condition positively [47]. The results of our study indicated that halotolerant L. adecarboxylata MO1 could reprogram plants under salinity stress to improve their growth and provide resistance via ACC deaminase synthesis and IAA production which significantly modulate plants endogenous sugar, organic acids, amino acids and stress responsive ABA. These results will guide the necessary future studies for tomato cultivation in areas where salinity is a major constraint. However, further research is required to validate the effectiveness of this PGPR isolate before recommendation for large scale at the field level.

Isolation
Soil samples having T-N 0.171%, P 2 O 5 0.026%, K 0.029 ppm, Na 0.050 ppm, Ca 0.057 ppm and Mg 0.094 ppm were collected from Gyeongbuk province in South Korea for isolation of bacteria from rhizosphere of tomato according to the method described by Kang et al. [65]. Briefly, collected soil samples (10 g) were transferred to a 250 mL flask containing 100 mL Amies solution [69] and then serially diluted to 1 × 10 − 4 . Then, a 0.1 mL suspension was spread on tryptic soy agar plates (TSA; Merck Co., Germany) and incubated at 28°C for 24 h. The incubated plates were examined every 6 h to assess bacterial growth, and the newly appeared bacteria were re-streaked on new plates to get obtain colonies. Single pure colonies were cultured in LB (Luria-Bertani) broth.

Selection of effective isolate
The initial screening for halotolerant IAA and ACC deaminase producing bacteria was carried out according to the method described by Shahzad et al. [24]. Briefly, 1 mL Salkowski reagent was added to 1 mL culture filtrate Bars with different letters indicate significant differences (P ≤ 0.05) among treatments based on DMRT. Each value represents the mean ± SD of six replicates from each of three independent experiments of bacterial isolates. Among the various bacterial isolates, MO1 changed the color to pink, indicating that MO1 produced IAA [68].
Moreover, presence of ACC deaminase responsible gene 'acdS' were examined by PCR analysis using (5′-3′) primers (Forward: ATCGGCGGCATCCAGWSNAAY-CANAC and Reverse: GTGCATCGACTTGCCCTCRTA-NACNGGRT) as describe by Wang et al. [70]. Briefly, PCR was carried out for 35 cycles with the initial denaturation at 94°C for 3 min, cyclic denaturation at 94°C for 30 s, annealing 58°C for 30 s and extension at 72°C for 2 min with a final extension of 7 min at 72°C using 50 μL reaction mixture containing 50 ng of DNA, 20 pmoles of each primer, 1.25 units of Taq DNA polymerase, 200 μM of each dNTPs and 1× PCR buffer. Moreover the PCR product was examined by agarose gel electrophoresis.
In addition, the salinity tolerance potential of MO1 in a saline environment was investigated according to the method described by Shahzad et al. [24]. Briefly, MO1 was grown in three concentrations of NaCl (120, 250, and 500 mM) in LB media (10 g tryptone, 5 g yeast extract, 10 g NaCl, pH 7.0 ± 0.2, autoclaved for 15 min at 121°C) to determine its growth dynamics and salinity tolerance capability. Growth dynamics were assessed using bacterial cell density at OD 600 (T60 UV VIS Spectrophotometer).

IAA quantification by GC/MS
IAA produced by MO1 in culture broth without L-Trp was extracted and analyzed by GC/MS with selected ion monitoring (SIM; 6890 N network GC system and 5973 network mass selective detector; Agilent Technologies, Santa Clara, CA, USA). The GC/MS conditions used for IAA quantification are given in the supplementary table (Additional file 1: Table S1) according to the method described by Shahzad et al. [40].

Salinity stress application and PGPR inoculation
A plant microbe interaction experiment was carried out to confirm the growth-promoting and stress-mitigating potential of the isolated L. adecarboxylata MOI. The experiment was carried out in complete randomized design with six treatments (Control, MO1, and GB each with and without 120 mM NaCl) in triplicate. Tomato (Solanum lycopersicum L. 'Yegwang') seeds were purchased from Danong Co. (Korea) and surface sterilized with 70% EtoH followed by 2.5% NaOH and rinsed with deionized distilled water and were germinated at 28°C in an incubator. Equal-sized seedlings (1 seedling per pot) were moved to sterilized plastic pots (10 cm width and 9 cm height) filled with 500 mg autoclaved horticultural substrate composed of peat moss (10-15%), coco peat (45-50%), perlite (35-40%), and zeolite (6-8%) with the NO 3 − (∼0.205 mg g − 1 ), NH 4+ (∼0.09 mg g − 1 ), P 2 O 5 (∼0.35 mg g − 1 ), and K 2 O (∼0.1 mg g − 1 ). Moreover, the soil pH was 5-7, bulk density was under 0.3 mg/m 3 , and EC (dS/m) was ≤1.2 [19]. The pots were placed in a growth chamber with a fixed program (day/night cycle: 12 h at 24°C, 12 h at 20°C; relative humidity: 65-70%; 1000 μE m − 2 s − 1 from sodium lamps). After two weeks, the pots were divided into six group according to the experimental design with 30 plants per treatment in triplicate. The plants were inoculated with 20 mL MO1 (4 × 10 8 cells mL − 1 ) and treated with GB followed by 50 mL 120 mM NaCl for 10 days on a daily basis to induce salinity-induced osmotic stress [72,73]. Upon stress completion (10 days) at vegetative growth stage, growth attributes (shoot and root length, shoot and root fresh weight, stem diameter, and chlorophyll content) were recorded and the plants were immediately harvested in liquid nitrogen and stored in − 80°C until further biochemical analyses.

Chlorophyll content assessment
Chlorophyll content was examined using a CCM-300 Chlorophyll Content Meter (Opti-Sciences, Inc., Hudson, NH, USA) as previously described by Kim et al. [74].

Sugar quantification
The contents of soluble sugars such as glucose, sucrose, and fructose were estimated according to the method described by Khan et al. [75]. Briefly, 0.5 g freeze dried grinded samples were extracted with 80% ethanol followed by vacuum drying. The dried residue was re-dissolved in 1 mL deionized water and passed through 0.45 μm Nylon-66 syringe filters. Furthermore, the filtered samples were injected to HPLC (Millipore Co., Waters Chromatography, Milford, MA, USA).

Organic acid estimation
Organic acids were measured according to the method described by Bilal et al. [76] . Briefly, 0.1 g of freeze dried grinded samples were added to 9 mL of distilled water and left overnight at room temperature. Then the sample was passed through a 0.22 μm syringe filter and 20 μL using HPLC on a model 600E system (Waters, Millford, MA, USA) equipped with a refractive index detector (RI, Model 410) with fixed isocratic conditions (mobile phase: 0.005 M H 2 SO 4 in water, flow rate 0.6 mL min − 1 , column temperature 65°C, PL Hi-Plex H column).

Amino acid determination
Amino acids were determined according to the method described by Shahzad et al. [77]. Briefly, freeze dried plant samples were hydrolyzed in 6 N HCl under vacuum in 4 mL tubes at 110°C for 24 h, followed by 80°C for 24 h. The dried residue was homogenized in 0.02 N HCl and was passed through 0.45-μm filter. Amino acids were quantified by an automatic amino acid analyzer (HITACHI L-8900, Japan) attached to a HITACHI HPLC system (packed column with ion-exchanging resin, No. 2622 PF; 4.6 × 60 mm) and ultraviolet detector (VIS1: 570 nm, VIS2: 440 nm).

ABA quantification
ABA was analyzed by GCMS (6890 N network GC system and 5973 network mass selective detector, Agilent Technologies, Palo Alto, CA, USA) according to the method described by Qi et al. [78]. Moreover, [(±)-3,5,5,7,7,7-d 6 ]-ABA was added as internal standard and for quantification, the Lab-Base (ThermoQuset, Manchester, UK) data system software was used to monitor responses to ions of m/e 162 and 190 for Me-ABA Me-[ 2 H 6 ]-ABA and m/z 166 and 194 for Me-[2H6]-ABA.

Statistical analysis
The data which are presented as means ± standard deviation were collected and pooled from triplicate treatments from each of three independently repeated experiments and were subjected to Duncan's multiple range test (DMRT) using SAS version 9.2 (Cary, NC, USA). Graphs were constructed using GraphPad prism. Moreover, the biochemical data obtained from each repetition were pooled together for their respective treatment and grouped into normal and stress conditions. A two-way analysis of variance (ANOVA) was performed to determine the significance level (P < 0.05). The two way ANOVA table is given in the Additional file 2: Table S2.

Additional files
Additional file 1: Table S1. GC/MS -SIM conditions used for analysis and quantification of the indole-3-acetic acid. (DOCX 12 kb) Additional file 2: Table S2. Two-way ANOVA table of the biochemical analysis performed for tomato plants with and without MO1 inoculation and GB treatment under normal and 120 mM NaCl stress. (DOCX 18 kb) Abbreviations ABA: Abscisic acid; GA: Gibberellins.; GB: Glycine betaine; IAA: Indole-3-acetic acid