Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1

Background The PII protein family comprises homotrimeric proteins which act as transducers of the cellular nitrogen and carbon status in prokaryotes and plants. In Herbaspirillum seropedicae, two PII-like proteins (GlnB and GlnK), encoded by the genes glnB and glnK, were identified. The glnB gene is monocistronic and its expression is constitutive, while glnK is located in the nlmAglnKamtB operon and is expressed under nitrogen-limiting conditions. Results In order to determine the involvement of the H. seropedicae glnB and glnK gene products in nitrogen fixation, a series of mutant strains were constructed and characterized. The glnK- mutants were deficient in nitrogen fixation and they were complemented by plasmids expressing the GlnK protein or an N-truncated form of NifA. The nitrogenase post-translational control by ammonium was studied and the results showed that the glnK mutant is partially defective in nitrogenase inactivation upon addition of ammonium while the glnB mutant has a wild-type phenotype. Conclusions Our results indicate that GlnK is mainly responsible for NifA activity regulation and ammonium-dependent post-translational regulation of nitrogenase in H. seropedicae.


Background
The PII family comprises homotrimeric proteins that have important roles in the control of the central metabolism in bacteria and plants, acting as transducers of the cellular nitrogen and carbon levels [1,2]. In many Proteobacteria studied there is a pair of PII proteins, usually called GlnB and GlnK, and their function is to sense the cellular levels of nitrogen, carbon and energy by binding the effectors 2-oxoglutarate, ATP and ADP [2,3]. These signals are then relayed to target proteins through conformational changes triggered by interaction with the effectors. The proteobacterial PII proteins also undergo a cycle of uridylylation/deuridylylation catalyzed by the bifunctional GlnD protein [1] in response to the intracellular levels of nitrogen. These conformational and covalent state changes stimulate or inhibit interactions of PII with different cellular protein targets involved in nitrogen and carbon metabolism [2].
PII proteins are key players in the regulation of nitrogen fixation in Proteobacteria. In Klebsiella pneumoniae and Azotobacter vinelandii, GlnK is required to regulate the activity of NifL, which inhibits NifA, the nif gene specific activator, under nitrogen-excess conditions [4][5][6]. In Azospirillum brasilense and Rhodospirillum rubrum GlnB is necessary for the activation of NifA under nitrogen-limiting conditions [7][8][9], whereas in Rhodobacter capsulatus both PII proteins are necessary for the NH 4 + -dependent regulation of NifA activity [10]. In addition, PII proteins are also involved in the post-translational control of nitrogenase activity in R. rubrum [11] and in A. brasilense through interaction with DraT, DraG and AmtB [12].
Herbaspirillum seropedicae is a nitrogen-fixing b-Proteobacterium isolated from the rhizosphere and tissues of several plants, including economically important species [13]. In this organism two PII-like coding genes were identified, glnB and glnK [14,15]. The glnB gene is monocistronic and its expression is constitutive [14], whereas glnK is apparently co-transcribed with amtB and orf1, which encode for an ammonium transporter and a membrane associated protein of unknown function, respectively [15]. Recently orf1 was named nlmA (nitrogen limitation membrane protein A) since its product was detected in membrane extracts of H. seropedicae grown under nitrogen-limitation conditions [16]. The expression of the nlmAglnKamtB operon is dramatically increased under nitrogen-limiting conditions and is dependent on NtrC [15]. As in other Proteobacteria, both PII proteins from H. seropedicae are targets of covalent modification by GlnD (uridylyltransferase/uridylyl removing enzyme) in response to the levels of ammonium ions [17].

Results and Discussion
To analyze the role of GlnK and GlnB in the control of nitrogen fixation in H. seropedicae, glnB (LNglnB) and glnK (LNglnK) insertional mutants and a glnK in-frame deletion mutant strain (LNglnKdel) were constructed and their phenotypes analyzed under different physiological conditions. These mutant strains were able to grow using nitrate as sole nitrogen source (data not shown).
The effect of glnB and glnK disruption on the NtrCdependent expression of the nlmAglnKamtB operon [15] was determined using chromosomal amtB::lacZ transcriptional fusions of strains LNamtBlacZ, LNglnBamtBlacZ and LNglnKamtBlacZ. These strains were grown under N-limiting (5 mmol/L glutamate or 2 mmol/L NH 4 Cl) or N-excess (20 mmol/L NH 4 Cl) conditions and assayed for β-galactosidase. The LNamtBlacZ strain grown under N-limiting conditions showed β-galactosidase activity 21 times higher than in high ammonium (Table 1), confirming that nlmAglnKamtB is highly expressed under N-limiting conditions [15]. Strains LNglnKamtBlacZ and LNglnBamtBlacZ revealed a similar pattern of amtB expression, indicating that the mutation of either glnK or glnB does not affect nlmAglnKamtB expression. Since nlmAglnKamtB transcription is NtrC-dependent, these results suggest that GlnB and GlnK can substitute for each other in control of the NtrC/NtrB system in H. seropedicae. In agreement with this suggestion, ntrC [18] and glnD (unpublished results) mutants strains of H. seropedicae are unable to grow on nitrate, whereas the glnB and glnK mutant strains can use nitrate as sole nitrogen source.
In Escherichia coli both GlnB and GlnK are involved in the regulation of NtrC phosphorylation by NtrB, although GlnB is more effective [19]. Although several attempts were made, we failed to construct a double glnBglnK mutant suggesting that an essential role is shared by these proteins in H. seropedicae.
The effect of glnK or glnB mutation on nitrogenase activity of H. seropedicae was determined in cultures grown in NH 4 + -free semi-solid NFbHP medium ( Figure 1). Nitrogenase activity was reduced by approximately 95% in both glnK strains (LNglnKdel and LNglnK) indicating that GlnK is required for nitrogenase activity in H. seropedicae. On the other hand, the glnB strain (LNglnB) showed activity similar to that of the wild-type. These results contrast with those reported by Benelli et al [14] who constructed a H. seropedicae glnB::Tn5-20B mutant (strain B12-27) that was unable to fix nitrogen. Immunoblot assays did not detect GlnK in the B12-27 strain [Additional file 1: Supplemental Figure S1], suggesting that a secondary recombination event may have happened in this strain resulting in loss of GlnK not observed by Benelli et al [14].
The nitrogenase phenotype of the glnK mutants was complemented by pLNOGA (nlmAglnKamtB) and also partially restored (about 50%) by a plasmid expressing glnB under control of its own promoter (pACB210) suggesting that a higher copy number of glnB can substitute for glnK under N-limitation. The lower nitrogenase activity of the glnK strains could be due to lack of nif expression or inhibition of nitrogenase. We therefore analyzed the effect of the glnK mutation on the NtrC-dependent nifA promoter [20] and on the NifAdependent nifB promoter of H. seropedicae [21] by using plasmids carrying nifA::lacZ (pRW1) or nifB::lacZ (pEMS140) fusions ( Table 2). The β-galactosidase activity was the same in both wild-type (SmR1) and glnK (LNglnK) strains containing nifA::lacZ, supporting the view that GlnK is not strictly necessary for NtrC regulation in H. seropedicae in the presence of a functional glnB gene. On the other hand, expression of the nifB:: lacZ fusion was reduced 10-fold in the glnK mutant compared to the wild-type, indicating that GlnK is required for nifB expression in H. seropedicae, even in the presence of wild type glnB. These results indicate that the lower nitrogenase activity in the glnK mutants was the result of lack of nif expression, most likely due to impaired NifA activity. Previous results showed that the N-terminal domain of H. seropedicae NifA is required for controlling its activity in response to NH 4 + , and that an N-truncated form of NifA is transcriptionally active, but not responsive to NH 4 + levels [22,23]. Thus, the nitrogenase activity was determined in the glnK mutants carrying pRAMM1 or pLNΔNifA which express a full NifA and an N-truncated form of NifA, respectively ( Figure 1). The nitrogenase activity of the glnK mutants was restored only by the N-truncated-NifA protein, reinforcing the indication that the nitrogenase negative phenotype of glnK strain is due to the presence of an inactive NifA.
Nitrogenase activity is reversibly inhibited by addition of ammonium or energy depletion in several diazotrophs, a phenomenon called nitrogenase switch-off. The best studied process is the reversible NifH ADP-ribosylation carried out by the DraT and DraG enzymes whose activities are controlled by processes involving PII proteins at least in some diazotrophs [11,12,24,25]. A PII protein has also been implicated in the control of nitrogenase by direct interaction with NifH in the methanogenic archaeon Methanococcus maripaludis [26]. In H. seropedicae reversible ADP-ribosylation of NifH by the DraT/ DraG does not occur since draTG genes are absent [27] [GenBank:CP002039]. Although the mechanism of NH 4 + -dependent nitrogenase control in this organism is not known, it is thought to be due to change in prevailing physiological conditions leading to nitrogenase inhibition. Since the glnK mutant is Nif -, we used strain LNglnKdel carrying plasmid pLNΔNifA for the switch-off experiments. Addition of low concentrations of NH 4 Cl (300 μmol/L) to derepressed cells caused an inactivation of nitrogenase ( Figure 2A). Wild-type and glnB strains retained less than 20% of initial nitrogenase activity 25 minutes after ammonium addition, which was restored to 60-70% of initial activity 60 minutes after ammonium addition. This effect does not involve protein synthesis since the presence of chloramphenicol or tetracycline had no effect on this behavior [28]. Although nitrogenase of strain LNglnKdel/pLNΔNifA was partially inhibited by ammonium addition, the strain retained about 50% of its initial activity, indicating only a partial nitrogenase switch-off ( Figure 2A). After addition of 1 mmol/L of NH 4 Cl ( Figure 2B) the activity of the wild-type and glnB strains dropped sharply to less than 30% and did not recover even 120 minutes after ammonium addition. In contrast, 40% of the initial nitrogenase activity was still present in the glnK strain 120 minutes after ammonium addition and the decrease in nitrogenase activity was slower: 20 minutes after ammonium addition the wildtype had only 25% activity, whereas the glnK strain had about 65% of the original nitrogenase activity. These results indicate that GlnK is involved in the nitrogenase inactivation by NH 4 + in H. seropedicae, and that GlnB cannot fully replace GlnK in triggering nitrogenase switch-off. It is interesting to note that there was also a
Values are averages of at least three independent experiments ± standard deviation delay in nitrogenase reactivation in the glnK mutant (Figure 2A), which may suggest that GlnK is involved in both nitrogenase inactivation by NH 4 + and re-activation upon NH 4 + depletion. Recently results using a proteomic approach [16] showed that H. seropedicae GlnK is associated with the membrane at higher concentration immediately after addition of ammonium. This membrane association was shown to be AmtB-dependent, as shown in several bacteria [2,16]. Previous results also showed that an amtB mutant has a partial NH 4 + switch off very similar to that shown by the glnK mutant [15]. These results allow us to propose a model for the regulation of nitrogen fixation in H. seropedicae. Under N-limiting conditions, NtrC-dependent promoters are activated leading to expression of nifA and nlmAglnKamtB genes. The status of fixed nitrogen is signaled to NtrC via the uridylylation state of either GlnB or GlnK. Under a low ammonium and oxygen condition, NifA activates the expression of nif genes in a process which requires GlnK, most probably in an uridylylated form. Thus, under N-limiting conditions the nitrogenase complex is active, AmtB is associated with the membrane, NlmA is most probably in the periplasm and GlnK is mainly located in the cytoplasm. When ammonium is added, deuridylylated GlnK rapidly associates with the cell membrane by interacting with AmtB to form the GlnK-AmtB complex which, in turn, signals to nitrogenase to switch-off by a yet unknown process.

Conclusions
In summary, our results show that both GlnB and GlnK proteins can regulate NtrC-dependent promoters in H. seropedicae. Under physiological conditions, GlnK is required for NifA activity control. GlnK also controls the nitrogenase switch-off in response to NH 4 + by a mechanism which most probably involves the formation of a membrane-bound GlnK-AmtB complex.
Enzyme assays β-galactosidase activity was determined in cells carrying a lacZ fusion as described [31]. To study the amtB-lacZ-Km R chromosomal fusion expression, H. seropedicae strains carrying chromosomal transcriptional fusions were grown for 14 hours in NFbHP medium containing glutamate (5 mmol/L) or NH 4 Cl (2 mmol/L or 20 mmol/L), and assayed for β-galactosidase activity. To study the nifA and nifB expression, H. seropedicae strains carrying plasmid-borne transcriptional fusions nifA::lacZ or nifB::lacZ were grown for 14 hours in NFbHP medium containing NH 4 Cl (10 mmol/L) under Protein concentration was determined by the Bradford method [32] using bovine serum albumin as standard.

Construction of the LNglnB mutant of H. seropedicae
Plasmid HS26-FP-00-000-021-E03 (Genopar consortium, http://www.genopar.org), which contains the H. seropedicae glnB gene in pUC18, was linearized with EcoRI and treated with T4DNA polymerase. It was then digested with HindIII to release a 1.7 kb fragment containing the glnB gene. This fragment was subcloned into the vector pSUP202 previously linearized with BamHI, treated with T4DNA polymerase and digested with Hin-dIII to produce plasmid pACB192.
In vitro transposon mutagenesis of the glnB gene carried by plasmid pACB192 was performed using the EZ:: TN™ <TET-1> Insertion Kit (Epicentre Technologies) following the manufacturer's instructions. A plasmid containing the transposon insertion in the glnB coding region was selected and named pACB194. This plasmid was introduced by conjugation to H. seropedicae SmR1 using E. coli strain S17.1 as the donor. Recombinant colonies were selected for tetracycline resistance and screened for the loss of chloramphenicol resistance (vector marker). Southern blot of restriction enzyme digested genomic DNA was used to confirm the presence of the transposon in the glnB gene (data not shown). This H. seropedicae glnB-Tc R strain was named LNglnB.

Construction of the LNglnK mutant of H. seropedicae
To clone the glnK gene, chromosomal DNA of H. seropedicae was amplified using the primers glnKD (5'-GACT-GAAAGGATCCGCGTGTCC-3', BamHI restriction site is underlined) and glnKR (5'-CGAGGGCAAAGCTT CTTCGGTGG-3', HindIII restriction site is underlined). The amplified fragment was then ligated into BamHI/ HindIII-cut pTZ18R, generating the plasmid pLNglnK. This BamHI/HindIII fragment containing the glnK gene was then subcloned into pSUP202, yielding plasmid pSUPglnK. A sacB-Km R cassette excised with BamHI from pMH1701 [35] was inserted into the BglII site of the glnK gene. The resulting plasmid (pSUPglnKsacB) was transferred into H. seropedicae SmR1 by conjugation using E. coli strain S17.1 as the donor. Mutant colonies were selected for kanamycin resistance and screened for the loss of chloramphenicol resistance, as before. Hybridization of genomic DNA was used to confirm the presence of the cassette in the glnK gene (data not shown). This glnK-Km R mutant was named LNglnK.

Construction of the LNglnKdel mutant of H. seropedicae
To construct a mutant containing an in-frame 192 bp deletion of the glnK gene, plasmid pUCG08del containing the 3' terminus of the nlmA gene, the glnK gene and the 5' terminus of the amtB gene was used as a template in two distinct PCR reactions. Primers M13universal and GlnKdelR (5' AAGCCCTCGAGTTCAGTCACGGT 3', XhoI restriction site is underlined) were used to amplify a 180 bp region upstream of glnK and the first 107 bp of the glnK gene. The primers M13reverse and GlnKdelD (5' GGACCTGCTCGAGGTGATCCGT 3', XhoI restriction site is underlined) were used to amplify the last 58 bp of the glnK gene and the first 180 bp of amtB. The amplified fragments were joined by the XhoI sites. This fragment containing glnK deleted of 192 bp was then used as template for a PCR reaction with the primers M13reverse and M13universal. The resulting PCR product was digested with BamHI and PstI and inserted into pUC18 to give pUCglnKdel. This fragment was then subcloned into pSUP202, yielding the plasmid pSUPglnKdel. A sacB-Km R cassette excised with BamHI from pMH1701 [35] was inserted into the vector region of the BamHI-cut pSUPglnKdel plasmid. The resulting plasmid (pSUPglnKdelsacB) was conjugated into H. seropedicae SmR1 using E. coli strain S17.1 as the donor. Recombinant colonies were selected for kanamycin and chloramphenicol resistance. One mutant strain was selected, and grown overnight in liquid NFbHP medium supplemented with ammonium chloride (20 mmol/L) and 80 μg/mL streptomycin. One microliter of the culture was plated on solid NFbHP medium supplemented with 20 mmol/L NH 4 Cl, 5% sucrose and 80 μg/mL streptomycin. Sucrose is toxic to bacteria containing the sacB gene in the chromosome, therefore only strains that lost the sacB-Km R cassette by a second homologous recombination event would grow. The selected strains were analyzed by PCR with the primers GlnKF1 (5'TGTCCAAGACCTTCGACG3') and GlnKR1 (5'CATGCTCATTAGAGTTCC3') which were homologous to the glnK flanking 5'-and 3'-regions, confirming the deletion of the 192 bp glnK fragment (data not shown). This in-frame glnK strain (ΔglnK) was named LNglnKdel.

Construction of plasmid pLNΔNifA
An Eco47III/SacI DNA fragment containing the nifA gene promoter region of H. seropedicae was excised from the plasmid pEMS301 [36] and sub-cloned into the SmaI/SacI-cut vector pDK6 [37], yielding plasmid pDK6pnifA. An XbaI DNA fragment encoding for the central and C-terminal region of NifA protein (ΔN-NifA) of H. seropedicae was excised from the plasmid pRAM2T7 and sub-cloned into the XbaI-cut pDK6pnifA, in the same orientation as the nifA promoter, yielding plasmid pDK6nifACT. Finally, a SacI/Hin-dIII DNA fragment containing the nifA 5'-truncated gene was excised from pDK6nifACT and sub-cloned into pLAFR3.18Cm digested with SacI and HindIII. The generated plasmid was named pLNΔNifA and encodes for the central and C-terminal domains of NifA under control of the nifA promoter.

Construction of chromosomal amtB::lacZ transcriptional fusions
To construct amtB-lacZ transcriptional fusions, the suicide plasmid pSUPamtBClacZ was introduced by conjugation, using E. coli strain S17.1 as the donor, into H. seropedicae strains SmR1, LNglnKdel and LNglnB resulting in the strains LNamtBlacZ, LNglnKamtBlacZ and LNglnBamtBlacZ, respectively. Genomic DNA hybridization confirmed the presence of the cassette lacZ-Km R in the amtB gene (data not shown).

Additional material
Additional file 1: Immunoblot analysis of H. seropedicae PII proteins.

List of Abbreviations
Ap R : ampicillin resistance; Cm R : chloramphenicol resistance, Km R : kanamycin resistance; Tc R : tetracycline resistance; Sm R : streptomycin resistance.