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  1. PCR amplification of bacterial 16S rRNA genes provides the most comprehensive and flexible means of sampling bacterial communities. Sequence analysis of these cloned fragments can provide a qualitative and qua...

    Authors: Elena Busti, Roberta Bordoni, Bianca Castiglioni, Paolo Monciardini, Margherita Sosio, Stefano Donadio, Clarissa Consolandi, Luigi Rossi Bernardi, Cristina Battaglia and Gianluca De Bellis

    Citation: BMC Microbiology 2002 2:27

    Content type: Methodology article

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  2. The aberrant cell-cycle progression of HIV-1-infected kidney cells plays a major role in the pathogenesis of HIV-associated nephropathy, however the mechanisms whereby HIV-1 induces infected glomerular podocyt...

    Authors: Peter J Nelson, Masaaki Sunamoto, Mohammad Husain and Irwin H Gelman

    Citation: BMC Microbiology 2002 2:26

    Content type: Research article

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  3. We have previously reported that altered culture conditions (a broth media with shaking) could induce a strain of Helicobacter pylori to assume a long spiral morphology resembling that described for Helicobacter ...

    Authors: Kathleen MB Vinette, Kathleen M Gibney, Roy Proujansky and Paul T Fawcett

    Citation: BMC Microbiology 2002 2:24

    Content type: Research article

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  4. Detecting microbial life in extraterrestrial locations is a goal of space exploration because of ecological and health concerns about possible contamination of other planets with earthly organisms, and vice ve...

    Authors: Ronald L Crawford, Andrzej Paszczynski, Qingyong Lang, Daniel P Erwin, Lisa Allenbach, Giancarlo Corti, Tony J Anderson, I Francis Cheng, Chien Wai, Bruce Barnes, Richard Wells, Touraj Assefi and Mohammad Mojarradi

    Citation: BMC Microbiology 2002 2:22

    Content type: Methodology article

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  5. The number of patients with yeast infection has increased during the last years. Also the variety of species of clinical importance has increased. Correct species identification is often important for efficien...

    Authors: Thierry De Baere, Geert Claeys, Danielle Swinne, Caroline Massonet, Gerda Verschraegen, An Muylaert and Mario Vaneechoutte

    Citation: BMC Microbiology 2002 2:21

    Content type: Methodology article

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  6. An avian papillomavirus genome has been cloned from a cutaneous exophytic papilloma from an African grey parrot (Psittacus erithacus). The nucleotide sequence, genome organization, and phylogenetic position of th...

    Authors: Ruth Tachezy, Annabel Rector, Marta Havelkova, Elke Wollants, Pierre Fiten, Ghislain Opdenakker, A Bennett Jenson, John P Sundberg and Marc Van Ranst

    Citation: BMC Microbiology 2002 2:19

    Content type: Research article

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  7. Insertion Sequence (IS) elements are mobile genetic elements widely distributed among bacteria. Their activities cause mutations, promoting genetic diversity and sometimes adaptation. Previous studies have exa...

    Authors: Dominique Schneider, Esther Duperchy, Joëlle Depeyrot, Evelyne Coursange, Richard E Lenski and Michel Blot

    Citation: BMC Microbiology 2002 2:18

    Content type: Research article

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  8. Chlamydia pneumoniae infection has been detected by serological methods, but PCR is gaining more interest. A number of different PCR assays have been developed and some are used in combination with serology for d...

    Authors: Tina Mygind, Svend Birkelund, Niels H Birkebæk, Lars Østergaard, Jørgen Skov Jensen and Gunna Christiansen

    Citation: BMC Microbiology 2002 2:17

    Content type: Research article

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  9. Since Mycobacterium avium subspecies paratuberculosis (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiol...

    Authors: Sabrina Corti and Roger Stephan

    Citation: BMC Microbiology 2002 2:15

    Content type: Research article

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  10. Methods for the extraction of DNA from filamentous fungi are frequently laborious and time consuming because most of the available protocols include maceration in liquid nitrogen after the mycelium has been gr...

    Authors: Alexandre Cassago, Rodrigo Alexandre Panepucci, Ana Maria Tortella Baião and Flavio Henrique-Silva

    Citation: BMC Microbiology 2002 2:14

    Content type: Methodology article

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  11. Escherichia coli can respire anaerobically using dimethyl sulfoxide (DMSO) or trimethylamine-N-oxide (TMAO) as the terminal electron acceptor for anaerobic energy generation. Expression of the dmsABC genes that e...

    Authors: Shawn MD Bearson, Jeffrey A Albrecht and Robert P Gunsalus

    Citation: BMC Microbiology 2002 2:13

    Content type: Research article

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  12. Prompt laboratory diagnosis of Herpes simplex virus (HSV) infection facilitates patient management and possible initiation of antiviral therapy. In our laboratory, which receives various specimen types for det...

    Authors: Julie Burrows, Andreas Nitsche, Belinda Bayly, Elise Walker, Geoff Higgins and Tuckweng Kok

    Citation: BMC Microbiology 2002 2:12

    Content type: Methodology article

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  13. Herpesviruses are not only infectious agents of worldwide distribution in humans, but have also been demonstrated in various non-human primates as well. Seventy-eight gibbons were subjected to serological test...

    Authors: Kamol Sakulwira, Apiradee Theamboonlers, Phingphol Charoonrut, Parntep Ratanakorn and Yong Poovorawan

    Citation: BMC Microbiology 2002 2:11

    Content type: Research article

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  14. In Southeast Asia, dengue viruses often co-circulate with other flaviviruses such as Japanese encephalitis virus, and due to the presence of shared antigenic epitopes it is often difficult to use serological m...

    Authors: Mary Jane Cardosa, Seok Mui Wang, Magdline Sia H Sum and Phaik Hooi Tio

    Citation: BMC Microbiology 2002 2:9

    Content type: Research article

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  15. Polyamine synthesis produces methylthioadenosine, which has to be disposed of. The cell recycles it into methionine through methylthioribose (MTR). Very little was known about MTR recycling for methionine salv...

    Authors: Agnieszka Sekowska and Antoine Danchin

    Citation: BMC Microbiology 2002 2:8

    Content type: Research article

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  16. Nontypeable Haemophilus influenzae is an important cause of otitis media in children and lower respiratory tract infection in adults with chronic obstructive pulmonary disease (COPD). Several lines of evidence su...

    Authors: Timothy F Murphy and Charmaine Kirkham

    Citation: BMC Microbiology 2002 2:7

    Content type: Research article

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  17. Enterohaemorrhagic Escherichia coli (EHEC) is considered to be common cause of haemorrhagic colitis (HC), thrombotic thrombocytopenic purpura and haemolytic uraemic syndrome (HUS) in humans. In a previous paper, ...

    Authors: Nora L Padola, Marcelo E Sanz, Paula MA Lucchesi, Jesús E Blanco, Jorge Blanco, Miguel Blanco, Analía I Etcheverría, Guillermo H Arroyo and Alberto E Parma

    Citation: BMC Microbiology 2002 2:6

    Content type: Research article

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  18. Seeds of the legume plant Lathyrus sativus, which is grown in arid and semi arid tropical regions, contain Diamino Propionic acid (DAP). DAP is a neurotoxin, which, when consumed, causes a disease called Lathy...

    Authors: KR Rupesh, PL PremKumar, Vasanth V Shiva Kumar and Seetharaman S Jayachandran

    Citation: BMC Microbiology 2002 2:5

    Content type: Research article

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  19. The development of DNA amplification for the direct detection of M. tuberculosis from clinical samples has been a major goal of clinical microbiology during the last ten years. However, the limited sensitivity of...

    Authors: Thierry De Baere, Ricardo de Mendonça, Geert Claeys, Gerda Verschraegen, Wouter Mijs, Rita Verhelst, Sylvianne Rottiers, Leen Van Simaey, Catharine De Ganck and Mario Vaneechoutte

    Citation: BMC Microbiology 2002 2:4

    Content type: Methodology article

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  20. Horses infected with Leptospira present several clinical disorders, one of them being recurrent uveitis. A common endpoint of equine recurrent uveitis is blindness. Serovar pomona has often been incriminated, alt...

    Authors: Paula MA Lucchesi, Alberto E Parma and Guillermo H Arroyo

    Citation: BMC Microbiology 2002 2:3

    Content type: Research article

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  21. When Bacillus subtilis is cultured in a complex fluid medium under conditions where cell separation is suppressed, populations of multicellular macrofibers arise that mature into ball-like structures. The final s...

    Authors: Neil H Mendelson, David Morales and John J Thwaites

    Citation: BMC Microbiology 2002 2:1

    Content type: Research article

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  22. Post-transcriptional gene silencing (PTGS) by short interfering RNA has opened up new directions in the phenotypic mutation of cellular genes. However, its efficacy on non-nuclear genes and its effect on the i...

    Authors: Vira Bitko and Sailen Barik

    Citation: BMC Microbiology 2001 1:34

    Content type: Research article

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  23. Streptolysin S (SLS), the oxygen-stable hemolysin of Streptococcus pyogenes, has recently been shown to be encoded by the sagA/pel gene. Mutants lacking expression of this gene were less virulent in a dermonecrot...

    Authors: Thomas H Eberhard, Darren D Sledjeski and Michael DP Boyle

    Citation: BMC Microbiology 2001 1:33

    Content type: Research article

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  24. The malarial parasite, Plasmodium falciparum (Pf), is responsible for nearly 2 million deaths worldwide. However, the mechanisms of cellular signaling in the parasite remain largely unknown. Recent discovery of a...

    Authors: Sean Dobson, Bratati Kar, Rajinder Kumar, Brian Adams and Sailen Barik

    Citation: BMC Microbiology 2001 1:31

    Content type: Research article

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  25. The routine determination of drug resistance in newly HIV-1 infected individuals documents a potential increase in the transmission of drug-resistant variants. Plasma samples from twenty seven therapy naive HI...

    Authors: Maria Carla Re, Paola Monari, Isabella Bon, Davide Gibellini, Francesca Vitone, Marco Borderi and Michele La Placa

    Citation: BMC Microbiology 2001 1:30

    Content type: Research article

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  26. Toward the goals of providing an additional vector to add to the armamentarium available to HIV vaccinologists and of creating a bivalent vaccine effective against dengue virus and HIV, we have attempted to cr...

    Authors: Xiaowu Pang, Mingjie Zhang and Andrew I Dayton

    Citation: BMC Microbiology 2001 1:28

    Content type: Research article

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  27. The ability of Mycobacterium tuberculosis to survive and replicate in macrophages is crucial for the mycobacterium's ability to infect the host and cause tuberculosis. To identify Mycobacterium tuberculosis genes...

    Authors: Barbara H Miller and Thomas M Shinnick

    Citation: BMC Microbiology 2001 1:26

    Content type: Research article

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  28. Diarrhoea caused by Escherichia coli is an important cause of infant morbidity and mortality in developing countries. Enteropathogenic Escherichia coli (EPEC) is considered one of the major causes of diarrhoea in...

    Authors: Andréa Nascimento de Araújo and Loreny Gimenes Giugliano

    Citation: BMC Microbiology 2001 1:25

    Content type: Research article

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  29. Norwalk virus causes outbreaks of acute non-bacterial gastroenteritis in humans. The virus capsid is composed of a single 60 kDa protein. In a previous study, the capsid protein of recombinant Norwalk virus ge...

    Authors: Tomoko Yoda, Yoshitake Terano, Yasuhiko Suzuki, Kenji Yamazaki, Isao Oishi, Tsuyoshi Kuzuguchi, Hiroyoshi Kawamoto, Etsuko Utagawa, Koichi Takino, Hajime Oda and Tadayoshi Shibata

    Citation: BMC Microbiology 2001 1:24

    Content type: Research article

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  30. Exopolysaccharides (EPSs) produced by lactic acid bacteria are important for the texture of fermented foods and have received a great deal of interest recently. However, the low production levels of EPSs in co...

    Authors: Fredrik Levander, Malin Svensson and Peter Rådström

    Citation: BMC Microbiology 2001 1:23

    Content type: Methodology article

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  31. Several bacterial pathogens express antihost factors that likely decrease both their maximal growth rate (due to metabolic costs) as well as their mortality rate (by neutralizing host defenses). The pathogenic...

    Authors: Sara Bartra, Peter Cherepanov, Åke Forsberg and Kurt Schesser

    Citation: BMC Microbiology 2001 1:22

    Content type: Research article

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  32. Ribosomal RNA molecules are widely used for phylogenetic and in situ identification of bacteria. Nevertheless, their use to distinguish microorganisms within a species is often restricted by the high degree of se...

    Authors: Wilhelm Schönhuber, Guenhael Le Bourhis, Josselyne Tremblay, Rudolf Amann and Saulius Kulakauskas

    Citation: BMC Microbiology 2001 1:20

    Content type: Research article

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  33. The role of lymphocytes in the specific defence against L. infantum has been well established, but the part played by polynuclear neutrophil (PN) cells in controlling visceral leishmaniasis was much less studied....

    Authors: Déborah Rousseau, Sylvie Demartino, Bernard Ferrua, Jean François Michiels, Fabienne Anjuère, Konstantina Fragaki, Yves Le Fichoux and Joanna Kubar

    Citation: BMC Microbiology 2001 1:17

    Content type: Research article

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  34. Methylthioadenosine, the main by-product of spermidine synthesis, is degraded in Bacillus subtilis as adenine and methylthioribose. The latter is an excellent sulfur source and the precursor of quorum-sensing sig...

    Authors: Agnieszka Sekowska, Laurence Mulard, Susanne Krogh, Jane KS Tse and Antoine Danchin

    Citation: BMC Microbiology 2001 1:15

    Content type: Research article

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  35. Norwalk-like viruses are the most common cause of gastroenteritis outbreaks and sporadic cases of vomiting and diarrhoea. In healthy individuals infection is often mild and short-lived but in debilitated patie...

    Authors: Hugh J O'Neill, Conall McCaughey, Dorothy E Wyatt, Frederick Mitchell and Peter V Coyle

    Citation: BMC Microbiology 2001 1:14

    Content type: Research article

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  36. Enteropathogenic Campylobacter jejuni and C. coli are presently the most common cause of acute bacterial gastroenteritis in the developed world. An understanding of sources and means of transmission of Campylobac...

    Authors: John E Moore and Colin E Goldsmith

    Citation: BMC Microbiology 2001 1:13

    Content type: Methodology article

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