Fig. 1From: Symbiotic microbiome Staphylococcus epidermidis restricts IL-33 production in allergic nasal epithelium via limiting the cellular necroptosisExperimental protocol of intranasal inoculation withS. epidermidisin OVA-driven AR mouse model. (A) The experimental protocol for the development of AR mouse using BALB/C. Mice were sensitized by intraperitoneal injection of ovalbumin mixed with aluminum hydroxide on days 0, 7, and 14. Daily OVA intranasal challenge was performed from days 22 to 28 (OVA/OVA). Human nasal S. epidermidis (3.2 × 106 CFU/30 µl PBS) was inoculated at indicated time points (day 19, 20). (B) mRNA expression of femA gene, specific for S. epidermidis and normalized to cellular GAPDH transcript levels, were monitored by real-time PCR. (C) Colony counts of nasal lavage fluid were performedBack to article page