Fig. 5
Verification of the regulatory function of Brevundimonas sp. ArsR M. (A) Interactions between the ArsRM DBD and its potential DNA target. (B) Residues that interact with DNA; red and black labels represent residues of monomer 1 and monomer 2, respectively. (C) Oligonucleotide probes from upstream of arsRM. (D) ArsRM expression in E. coli BL21 (DE3). Lanes 1–2: unpurified total protein; 3–4: eluent from Ni-NTA column. Full-length gel is presented in Supplementary Figure S4. (E) Electromobility shift assays of ArsRM and ParsRM. Lanes ck1–ck3: probes ParsRM-1, ParsRM-2, and ParsRM-3, respectively, without ArsRM; lanes P1–P3: probes ParsRM-1, ParsRM-2, ParsRM-3, respectively, with ArsRM. The positions of the free (f) and shifted (s) probes are indicated by black and red arrows, respectively. Full-length gel is presented in Supplementary Figure S5