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Fig. 3 | BMC Microbiology

Fig. 3

From: Bifunctional protein ArsRM contributes to arsenite methylation and resistance in Brevundimonas sp. M20

Fig. 3

Construction of Brevundimonas sp. M20 ArsRM expression strain Escherichia coli ARM3. (A) Expression plasmid construction; lane M1: DL 15,000 DNA Ladder (Solarbio, M1700), 1: pET-arsRM digested by NdeI and XhoI, 2: arsR M fragment amplified by PCR, 3: pET-15b digested by NdeI and XhoI. Full-length gel is presented in Supplementary Figure S2. (B) PCR verification of E. coli strain ARM3; lane M2: D 15,000 + 2000 DNA Ladder (TIANGEN, MD116), 1–4: positive transformants, 5: blank control. Full-length gel is presented in Supplementary Figure S3

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BMC Microbiology

ISSN: 1471-2180

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