Detection of multidrug-resistant Shiga toxin-producing Escherichia coli in some food products and cattle faeces in Al-Sharkia, Egypt: one health menace

Table 5 Primer sequences designs for virulence genes of Shiga toxins; Stx1 and Stx2 in MDR E. coli O157 with the universal 16 s rRNA

Gene	Primer sequence (5'-3')	Length of amplified product	Reference
stx1	F- ACACTGGATGATCTCAGTGG R- CTGAATCCCCCTCCATTATG	614 bp	Dipineto et al., 2006 [76]
stx2	F- CCATGACAACGGACAGCAGT T R- CCTGTCAACTGAGCAGCACTTTG	779 bp
Universal 16 s rRNA	F- AGAGTTTGATCCTGGCTCAG	1038 bp	(Baron et al. 2013) [78]

MDR: multidrug- resistant, stx1: encodes Shiga toxin variant [Stx1], stx2: encodes Shiga toxin variant 2 [Stx2]

ISSN: 1471-2180