Fig. 4From: Genomic and functional characterization of carbapenem-resistant Klebsiella pneumoniae from hospital wastewaterSurvival assay of CRKP from HWW and clinical CRKP in the HWW during 50 days. (A) Growth trend of 11 CRKP from HWW. B Growth trend of 11 clinical CRKP. C Group analysis of growth proportion of every strain (compared to 0 day) between CRKP from HWW and clinical CRKP. 1 mL overnight bacterial culture (in LB broth) was suspended in duplicate in test tubes containing 40 mL of the autoclaved HWWs with shaking at 180 rpm. The number of bacteria was determined every 7 days in duplicate. Unpaired two-tailed Student’s t-tests and Wilcoxon sign rank test were performed to analyze the growth proportion of every strain (compared to 0 day) between CRKP from HWW and clinical CRKP. The error bars represent standard deviation (SD). Notes: NS: no significance, *: p < 0.05 and ***: p < 0.0001Back to article page