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Fig. 6 | BMC Microbiology

Fig. 6

From: Signatures in in vitro infection of NSC-34 mouse neurons and their cell nucleus with Rickettsia helvetica

Fig. 6

Protein and WB gel on R. helvetica infected and uninfected NSC-34 cells, respectively. Gels (A1) and (A2) represent the same whole gel where (A1) shows Cy5-labelled total protein expression showing the total distribution of protein in infected (+) and uninfected (-) NSC-34 cells during a 14-day period. For the timepoints illustrated in the figures the cells were grown in P-medium, except for Day 8 when cells are compared after growing in P- and D-medium respectively and (A2) WB analysis of the same membrane showing rickettsia proteins of R. helvetica infected (+) and uninfected (-) NSC-34 cells after 2 to 14 days using an anti-R. helvetica IgG antibody labeling of antigenic structures of the rickettsia bacteria. The expressed proteins increase over time and shows the same pattern in both P and D medium but were expressed in greater amount in D-medium which is illustrated Day 8 with both P- and D-medium in comparison. As seen after Day 10, one of the SPAs in size in the 97–225 kDa region was downregulated. (B) Represents six lanes of a Western blot membrane, where the specific anti-Sca4 protein antibody shows that protein expression increases in the infected (+) cells with time and that the downregulated R. helvetica protein in A2, after day 10, does not correspond to PS120 which remains expressed. Every other lane represents uninfected (-) NSC-34 cells. The original gels for A2 and B are included as Supplementary files

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