Fig. 2

DTT-induced Msm biofilms are rich in polysaccharides. Msm overexpressing GFP were exposed to 6 mM DTT for 29 h to induce biofilm formation. Biofilms were then stained with specific fluorophores, namely, (A) Nile red (1 mM) for staining the lipids, (B) Texas red Hydrazide (0.5 mg/ml) for staining polysaccharides, (C) lectin ConA (200 μg/ml) for staining the α-mannopyranosyl and α-glucopyranosyl-rich polysaccharides. Stained biofilms were then analyzed using CLSM (Scale bar: A and C-50 µm, B-200 µm). Lower panels in A-C show the 3-D image of the biofilms. Images are representative of three independent experiments