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Fig. 1 | BMC Microbiology

Fig. 1

From: Media component bovine serum albumin facilitates the formation of mycobacterial biofilms in response to reductive stress

Fig. 1

Formation of biofilm by Msm upon exposure to DTT: (A) (I) Culture of O.D.600–1.0 was exposed to 5 mM DTT (left panel). In control, no DTT was added to the culture (middle panel). To media alone, 5 mM DTT was added (right panel), (II) CV assays were performed on the samples described above. (B) (I) Msm cultures at an OD600 -1.0 were exposed to a 2 mM-8 mM range of DTT, and biofilm formation was recorded after 29 h of DTT exposure observed visibly, (II) quantified with CV assay. 4–6 mM of DTT leads to the formation of biofilm. (C) (I) Cultures of different O.D. (0.2 – 2) were exposed to 6 mM DTT, and biofilm formation was recorded after 29 h of DTT exposure observed visibly, (II) quantified with CV assay. Biofilm formation was observed at all O.D.s. (D) (I) Msm culture of OD600—1.0 was raised in a medium containing different concentrations of O-ADC (as indicated below the inkwell bottle). These cultures were exposed to DTT for 29 h and observed visibly, (II) quantified with CV assay. (E) (I) 7H9 medium was supplemented with reconstituted O-ADC having a variable concentration of BSA (concentration of BSA is denoted as % below the inkwell bottles), keeping the rest of the components constant. Bacterial culture was raised in these media and exposed to 6 mM DTT. Biofilm formation was facilitated in a BSA-dependent manner and was observed visibly, (II) quantified with CV assay. The column bar graphs were plotted using GraphPad Prism 8. Data represent mean ± SEM from 3 independent experiments. Statistical significance was determined using Student’s t-test, * indicates a p-value of < 0.05

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