Multi-factorial examination of amplicon sequencing workflows from sample preparation to bioinformatic analysis

BMC Microbiology

Table 1 Primers used in the multi-factorial study

Primer	Sequence (5’-3’)	*E. coli* positions^a	Variable regions	Amplicon size^a (nt)	Reference
357f	CCTACGGGAGGCAGCAG	344–360	V3-V4	432	Stackebrandt 1991 [59]
806r	GGACTACNVGGGTWTCTAAT	792–811	V3-V4	432	Apprill 2015 [60]
515f	GTGYCAGCMGCCGCGGTAA	520–538	V4-V4	254	Parada 2016 [61]
806r	GGACTACNVGGGTWTCTAAT	792–811	V4-V4	254	Apprill 2015 [60]
541f	GYCAGCMGCMGCGGTAATAC	522–541	V4-V5	373	Present study
914r	GYCCCCGTCWATTCMTTTGAGTTT	914–937	V4-V5	373	Present study

^aDetermined relative to the E. coli strain K-12 MG1655 16S rRNA gene sequence (GenBank accession number U00096)

ISSN: 1471-2180